ABOUT EPPENDORF
Stimulating Growth. Cultivating Solutions. – Expert Partner for Cell and Gene Therapy Development
Cell and gene therapy development requires large numbers of high-quality cells. Stirred-tank bioreactors facilitate efficient cell expansion when conventional 2D-culture systems reach their limits.
The Eppendorf Bioprocess Unit builds on decades of experience in upstream bioprocessing; since more than ten years Eppendorf is committed to contribute to the advancement of stem cell cultivation in stirred-tank bioreactors. The scalability, robustness, quality, and cost-effectiveness of cell culture processes are key to success when translating basic research to the development of commercially viable cell and gene therapies. Eppendorf supports you in developing powerful upstream bioprocesses for cell and gene therapy development.
Since 1945, the Eppendorf brand has been synonymous with customer-oriented processes and innovative products, such as laboratory devices and consumables for liquid handling, cell handling and sample handling. Today, Eppendorf and its more than 5000 employees serve as experts and advisors, using their unique knowledge and experience to support laboratories and research institutions around the world.
FEATURED CONTENT
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Reduce the time and risks involved in maintaining a traditional bioreactor with this single-use solution for biopharmaceutical manufacturers.
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Explore the growing field of cell and gene therapies along with a bioreactor system that is enabling researchers to expedite cell culture development and optimize their research time.
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Take a deeper look into innovative solutions for researchers facing challenges throughout the development of novel cell and gene therapeutics.
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By exploring innovative approaches to scaling up animal cell growth in cutting-edge bioreactor systems, foodtech companies are poised to offer healthier, safer, more sustainable fish for consumers.
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Review the distinct steps in the plasmid production workflow, along with tips for optimization from vector and strain selection to cultivation and beyond.
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This article collection highlights the importance of continued research and development in the field of bioprocessing to revolutionize the next generation of therapies and personalized medicine.
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Explore a study demonstrating the use of dielectric spectroscopy tools for monitoring expansion and differentiation of hiPSC aggregates in stirred-tank bioreactors.
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Application scientist, Dr. Jorge Escobar Ivirico, explains and underscores the key differences with the production of exosomes from other cellular therapies.
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Selecting the bioreactor that is best for your process and the dedicated space you have available requires consideration of many important factors.
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In this white paper we explain the key characteristics of stirred-tank bioreactors and which organisms are typically grown in them.
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This article examines some of the currently available schemes used in generating rAAV from suspension cultures, and describes what it takes to achieve scalable rAAV production.
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Great hopes and expectations are linked to stem cells as a tool for drug discovery and to stem cell-derived products in therapeutic applications. Though several products have made it to commercial stage, most of the research is still performed in small scales using simple cultivation systems such as spinner flasks or T-flasks.
CONTACT INFORMATION
Eppendorf SE
Bioprocess Center, Rudolf-Schulten-Straße 5
Jülich, 52428
GERMANY
PRODUCTS
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The SciVario twin is a future proof bioprocess controller, developed by Eppendorf, with the intuitive software, bringing the digital age to the laboratory.
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Whether your process includes cell culture or fermentation, autoclavable or single-use vessels, the BioFlo 320 seamlessly combines form and function in one state of the art package. A robust industrial design, intelligent sensors, Ethernet connectivity, and enhanced software capabilities are only a few of the features that set it apart from the competition. Combined with a sincere commitment to quality, the BioFlo 320 truly is the premium choice in bench-scale bioprocess control stations.
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Combine the benefits of single-use bioreactor technology with the reliable performance of conventional glass or stainless steel bioreactors – Discover the Eppendorf BioBLU product line.
BROCHURES
APPLICATION NOTES
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Review this demonstration of a bioreactor and bioreactor system combination used to isolate exosomes after culturing human adipose-derived stem cells in suspension on microcarriers.
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Explore the use of a mini bioreactor system for stem cell culturing that optimized process parameters of a human induced pluripotent stem cell (hiPSC) culture in a step-by-step process.
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Explore the differences between three cell culture bioprocesses using bench-scale CHO cells, and why tracking specific parameters is important to achieving the inoculation of bioreactors at high viability.
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Review this example of scalable AAV production on a suspension cell culture platform through the AAV-2 capsid production process in a controlled environment.
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Learn how stirred-tank bioreactors can be used to efficiently expand T cells from different donors.
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Explore the feasibility of plasmid DNA production using the DASbox Mini Bioreactor System and compare fed-batch with batch culture with respect to plasmid DNA yield and process efficiency.
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This study reviews a fully controlled system allowing an expansion of a high yield of viable cells and optimizing production of high-quality extracellular vesicles in standardized culture conditions.
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In this app note, we monitored and analyzed the metabolites derived from hiPSCs as well as cell adhesion and morphology, suggesting that we obtained an excellent physiological environment over time.
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Current bottlenecks in the production of LVs are caused mainly by the disadvantages of classical two-dimensional culture forms. Switching to bioreactors can eliminate those disadvantages and offer the benefits of process automation, tight regulation of production conditions, and reduced labor input.
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Effective drug discovery and development relies in large part on the availability of predictive preclinical model systems. Learn about a bioprocess for the large-scale manufacturing of cardiomyocytes derived from a variety of healthy and diseased hiPSC lines.
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Stem cell-derived cortical neurospheres are of great interest in basic neurobiology research and drug research. Cultivation in controlled, stirred-tank bioreactors can improve the homogeneity of neural spheroid cultures and allows culture scale-up.
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In this study, we successfully demonstrated large-scale culture of human adipose-derived mesenchymal stem cells (AdMSCs) in an industrial, single-use vessel at 3.75 L scale and demonstrate the fact that the process can be conveniently scaled up to industrial levels of production without having to rely on stainless steel culture facilities.
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The routine application of human pluripotent stem cells and their derivatives in regenerative medicine and innovative drug discovery will require the constant supply of high cell numbers in consistent, high quality.
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These promising culture systems allow efficient establishment and maintenance of cell aggregates, process monitoring and control, and process scale-up to larger volumes.
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This application note details the suitability of Eppendorf BioBLU 0.3c Single-Use Vessels controlled by a DASbox Mini Bioreactor System for the expansion of human bone marrow-derived mesenchymal stem cells on microcarriers.