Stem Cell Exosome Production On The SciVario® Twin

By Jorge L. Escobar Ivirico and Ma Sha, Eppendorf, Inc.,

Exosomes derived from mesenchymal stem cells (MSC) are a new alternative to restore tissues and organs, avoiding the limitations associated with stem cell therapy. MSC-derived exosomes are involved in MSC’s paracrine functions related to cell-to-cell communications and tissue reconstruction, leading to cell self-renewal and differentiation. Despite the benefits of stem cell use, there are limitations, including the large-scale production of cells that mimic physiological conditions. Therefore, increasing the mass production of exosomes in a controlled environment is the necessary next step. In this study, we used a combination of SciVario® twin bioreactor control system and BioBLU® 1c Single-Use Vessels as a model for large scale exosome production in bioreactors. The SciVario twin is a future-proof bioreactor control system developed by Eppendorf. Following the agile development principle, the controller was designed with a maximum working volume of 3.7 L at launch, but the flexibility of the system allows for future hard- and software updates using vessels as large as 40 L.

In this application note, we monitored and analyzed the metabolites derived from the human induced Pluripotent Stem Cells (hiPSC) as well as cell adhesion and  morphology, suggesting that we obtained an excellent physiological environment over time. Furthermore, we isolated, purified and quantified (by CD63 ELISA kit) the exosomes produced from MSC.