Application Note

Expansion Of Human Bone Marrow-Derived Mesenchymal Stem Cells In BioBLU 0.3c Single-Use Bioreactors

Source: Eppendorf

By Vincent Dufey, et al

Expansion Of Human Bone Marrow-Derived Mesenchymal Stem Cells In BioBLU 0.3c Single-Use Bioreactors

The use of adult stem cells holds great promise for new cell-based therapies and drug discovery. For their routine application, large numbers of cells have to be produced with consistently high quality. Two-dimensional cultivation systems, such as T-flasks, are widely used, but they are limited in terms of control and scalability. Stem cell expansion in rigid-wall, stirred-tank bioreactors, however, facilitates the precise control of critical process parameters like pH and dissolved oxygen, and allows a more straightforward scale-up to larger process dimensions. We tested the suitability of Eppendorf BioBLU 0.3c Single-Use Vessels controlled by a DASbox® Mini Bioreactor System for the expansion of human bone marrow-derived mesenchymal stem cells on microcarriers, and obtained 1 x 108 cells in a working volume of 250 mL. The cells were able to differentiate into osteocytes and chondrocytes, respectively, demonstrating that their expansion in stirred-tank bioreactors did not affect multipotency.

BioBLU Single-Use Vessels with maximum working volumes of 250 mL and 3.75 L were previously used for the expansion of human induced pluripotent stem cells as cell-only aggregates [1] and of adipose-derived mesenchymal stem cells on microcarriers [2]. These results, and our current data, suggest that Eppendorf BioBLU Single-Use Vessels are widely applicable for the expansion of different stem cell types at various scales.