Inline Monitoring Of hiPSC Growth And Differentiation

Hepatocyte-like cells derived from human-induced pluripotent stem cells (hiPSCs) hold great promise in drug screening and regenerative medicine applications. They can be obtained in high numbers by culturing 3D cell aggregates in stirred-tank bioreactors in a well-defined and controlled environment. As with other biomanufacturing processes, monitoring cell quality attributes is crucial. This study demonstrates the use of dielectric spectroscopy tools for monitoring expansion and differentiation of hiPSC aggregates in stirred-tank bioreactors.