Eppendorf Application Notes

  1. Achieve Fast And Efficient Isolation Of Exosomes From Stem Cells 10/5/2023

    Review this demonstration of a bioreactor and bioreactor system combination used to isolate exosomes after culturing human adipose-derived stem cells in suspension on microcarriers.

  2. Increasing iPSC Numbers Through Systematic Culture Process Optimization 10/5/2023

    Explore the use of a mini bioreactor system for stem cell culturing that optimized process parameters of a human induced pluripotent stem cell (hiPSC) culture in a step-by-step process.

  3. CHO Culture: Bioprocess Modes Batch Fed-Batch and Perfusion 2/2/2023

    Explore the differences between three cell culture bioprocesses using bench-scale CHO cells, and why tracking specific parameters is important to achieving the inoculation of bioreactors at high viability.

  4. Maximize Adeno-Associated Virus Production Using Suspension Culture 9/21/2022

    Review this example of scalable AAV production on a suspension cell culture platform through the AAV-2 capsid production process in a controlled environment.

  5. How To Best Optimize CD4 T Cells Expansion 9/21/2022

    Learn how stirred-tank bioreactors can be used to efficiently expand T cells from different donors.

  6. E. Coli Plasmid DNA Production 3/14/2022

    Explore the feasibility of plasmid DNA production using the DASbox Mini Bioreactor System and compare fed-batch with batch culture with respect to plasmid DNA yield and process efficiency.

  7. A Tool For Process Development, Stem-Cell Derived Exosome Production 3/10/2022

    This study reviews a fully controlled system allowing an expansion of a high yield of viable cells and optimizing production of high-quality extracellular vesicles in standardized culture conditions.

  8. Stem Cell Exosome Production On The SciVario® Twin 5/20/2021

    In this app note, we monitored and analyzed the metabolites derived from hiPSCs as well as cell adhesion and morphology, suggesting that we obtained an excellent physiological environment over time. 

  9. Transient Lentiviral Vector Production In HEK 293T Cells Using The BioFlo 320 Control Station With A BioBLU 5p Single-Use Packed-Bed Vessel 7/11/2019

    Current bottlenecks in the production of LVs are caused mainly by the disadvantages of classical two-dimensional culture forms. Switching to bioreactors can eliminate those disadvantages and offer the benefits of process automation, tight regulation of production conditions, and reduced labor input. 

  10. Controlled, Large-Scale Manufacturing Of hiPSC-Derived Cardiomyocytes In Stirred-Tank Bioreactors 6/10/2019

    Effective drug discovery and development relies in large part on the availability of predictive preclinical model systems. Learn about a bioprocess for the large-scale manufacturing of cardiomyocytes derived from a variety of healthy and diseased hiPSC lines.