Eppendorf Application Notes

  1. Maximize Adeno-Associated Virus Production Using Suspension Culture 9/21/2022

    Review this example of scalable AAV production on a suspension cell culture platform through the AAV-2 capsid production process in a controlled environment.

  2. How To Best Optimize CD4 T Cells Expansion 9/21/2022

    Learn how stirred-tank bioreactors can be used to efficiently expand T cells from different donors.

  3. E. Coli Plasmid DNA Production 3/14/2022

    Explore the feasibility of plasmid DNA production using the DASbox Mini Bioreactor System and compare fed-batch with batch culture with respect to plasmid DNA yield and process efficiency.

  4. A Tool For Process Development, Stem-Cell Derived Exosome Production 3/10/2022

    This study reviews a fully controlled system allowing an expansion of a high yield of viable cells and optimizing production of high-quality extracellular vesicles in standardized culture conditions.

  5. Stem Cell Exosome Production On The SciVario® Twin 5/20/2021

    In this app note, we monitored and analyzed the metabolites derived from hiPSCs as well as cell adhesion and morphology, suggesting that we obtained an excellent physiological environment over time. 

  6. Transient Lentiviral Vector Production In HEK 293T Cells Using The BioFlo 320 Control Station With A BioBLU 5p Single-Use Packed-Bed Vessel 7/11/2019

    Current bottlenecks in the production of LVs are caused mainly by the disadvantages of classical two-dimensional culture forms. Switching to bioreactors can eliminate those disadvantages and offer the benefits of process automation, tight regulation of production conditions, and reduced labor input. 

  7. Controlled, Large-Scale Manufacturing Of hiPSC-Derived Cardiomyocytes In Stirred-Tank Bioreactors 6/10/2019

    Effective drug discovery and development relies in large part on the availability of predictive preclinical model systems. Learn about a bioprocess for the large-scale manufacturing of cardiomyocytes derived from a variety of healthy and diseased hiPSC lines.

  8. Production Of Human iPSC-Derived Cortical Neurospheres In A Mini Bioreactor System 1/15/2019

    Stem cell-derived cortical neurospheres are of great interest in basic neurobiology research and drug research. Cultivation in controlled, stirred-tank bioreactors can improve the homogeneity of neural spheroid cultures and allows culture scale-up. 

  9. Billion-Cell Hypoxic Expansion Of Human Mesenchymal Stem Cells In BioBLU® 5c Single-Use Vessels 12/18/2018

    In this study, we successfully demonstrated large-scale culture of human adipose-derived mesenchymal stem cells (AdMSCs) in an industrial, single-use vessel at 3.75 L scale and demonstrate the fact that the process can  be conveniently scaled up to industrial levels of production without having to rely on stainless steel culture facilities.

  10. Culture Of 3D Cell Aggregates In Perfusion In A Mini Bioreactor System 5/8/2018

    These promising culture systems allow efficient establishment and maintenance of cell aggregates, process monitoring and control, and process scale-up to larger volumes.