Eppendorf Application Notes

  1. Transient Lentiviral Vector Production In HEK 293T Cells Using The BioFlo 320 Control Station With A BioBLU 5p Single-Use Packed-Bed Vessel 7/11/2019

    Current bottlenecks in the production of LVs are caused mainly by the disadvantages of classical two-dimensional culture forms. Switching to bioreactors can eliminate those disadvantages and offer the benefits of process automation, tight regulation of production conditions, and reduced labor input. 

  2. Controlled, Large-Scale Manufacturing Of hiPSC-Derived Cardiomyocytes In Stirred-Tank Bioreactors 6/10/2019

    Effective drug discovery and development relies in large part on the availability of predictive preclinical model systems. Learn about a bioprocess for the large-scale manufacturing of cardiomyocytes derived from a variety of healthy and diseased hiPSC lines.

  3. Production Of Human iPSC-Derived Cortical Neurospheres In A Mini Bioreactor System 1/15/2019

    Stem cell-derived cortical neurospheres are of great interest in basic neurobiology research and drug research. Cultivation in controlled, stirred-tank bioreactors can improve the homogeneity of neural spheroid cultures and allows culture scale-up. 

  4. Billion-Cell Hypoxic Expansion Of Human Mesenchymal Stem Cells In BioBLU® 5c Single-Use Vessels 12/18/2018

    In this study, we successfully demonstrated large-scale culture of human adipose-derived mesenchymal stem cells (AdMSCs) in an industrial, single-use vessel at 3.75 L scale and demonstrate the fact that the process can  be conveniently scaled up to industrial levels of production without having to rely on stainless steel culture facilities.

  5. Scalable Expansion Of Human Pluripotent Stem Cells 5/8/2018

    The routine application of human pluripotent stem cells and their derivatives in regenerative medicine and innovative drug discovery will require the constant supply of high cell numbers in consistent, high quality.

  6. Culture Of 3D Cell Aggregates In Perfusion In A Mini Bioreactor System 5/8/2018

    These promising culture systems allow efficient establishment and maintenance of cell aggregates, process monitoring and control, and process scale-up to larger volumes.

  7. Expansion Of Human Bone Marrow-Derived Mesenchymal Stem Cells In Single-Use Bioreactors 5/8/2018

    This application note details the suitability of Eppendorf BioBLU 0.3c Single-Use Vessels controlled by a DASbox Mini Bioreactor System for the expansion of human bone marrow-derived mesenchymal stem cells on microcarriers.