Increasing iPSC Numbers Through Systematic Culture Process Optimization

By Felix Manstein, Kevin Ullmann, and Robert Zweigerdt

Human induced pluripotent stem cells (hiPSCs) are a powerful tool for innovative approaches, such as drug discovery, in vitro disease modelling, or regenerative therapies. The DASbox® Mini Bioreactors and the DASbox Mini Bioreactor System have been tested to demonstrate their ability to systematically optimize the process parameters of a hiPSC culture in a step-by-step process. Using this bioreactor system, multiple important growth parameters were adapted to increase cell numbers during a 7-day-incubation period.

Explore how, through the DASbox Mini Bioreactor System, hiPSC was cultured in a way that allowed them to retained their stem cell properties, as well as the ability to develop into tissues of the endo-, meso-, and ectoderm.