Application Note

A Beginner's Guide To Sf9 Culture In Stirred-Tank Bioreactors

Source: Eppendorf SE

By Marcus Deinstadt and Ma Sha

GettyImages-1142684567 cell culture

Sf9 insect cells are a popular expression platform for recombinant proteins due to their suspension culture capabilities, high growth rate, and post-translational modification abilities. This application note provides a comprehensive guide to culturing Sf9 cells and producing recombinant proteins using the baculovirus expression vector system in stirred-tank bioreactors. It details workflow steps from virus preparation and cell expansion in shake flasks to bioreactor culture and protein production.

Utilizing a BioFlo® 120 bioreactor and BioBLU® 3c Single-Use Bioreactors, the study successfully produced green fluorescent protein (GFP). To delve into the specifics of this robust protocol and optimize your Sf9 cell culture processes, read the full application note.

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Eppendorf SE