FEATURED CONTENT

• 8 Tips To Improve Compensation In Multicolor Flow Experiments

  Flow cytometry compensation is critical, for it directly impacts data accuracy and quality. Here, we share eight tips to improve compensation in multicolor flow experiments.

• 4 Ways Your Droplet Sorter May Harm Your Cells

  Learn the 4 physical conditions that occur during droplet sorting, which may keep your cells from being happy.

• Isolation Of Antigen Specific CD8+ T Cells Using A Microchip-Based Cell Sorter

  Here, we demonstrate the capacity of a specific bench top sorter to allow for completely aseptic sorting conditions by using the system to sort cancer antigen-specific cytotoxic T cells out of PBMCs.

• Isolation Of CD19 CAR T Cells Directly From Patient Samples

  Isolating fully functional CAR T cells requires high quality cell separation. See how we successfully and efficiently isolated CD19 CAR T cells with the MACSprep™ CD19 CAR MicroBead Kit, human.

• Flow Cytometry Analysis Of Chemokine Receptors Expressed On Human NK Cells

  Learn how the knowledge generated by a deep and thorough analysis of chemokine receptors on natural killer (NK) cells can be helpful to scientists studying certain cancers and diseases.

• Flow Cytometry Analysis Of Isolated NK Cells From Whole Blood

  Here, we describe the multicolor flow cytometry analysis necessary to study natural killer (NK) cell phenotype and the function of isolated NK cells from whole blood.

• Generation And Preclinical Applications Of Humanized Mouse Models

  Here, we show an automated experimental setup for human CD34+ HSC enrichment from umbilical cord blood for the successful generation of humanized mouse models.

• Effective Enrichment Of Human TILs

  Research is being done to study a new method for isolating TILs from human colon tumor xenografts that could be more efficient and effective than traditional methods.

• CD56 Cell Depletion From PBMCs Applied To Validate A HIV-Specific ADCC Mechanism

  Explore results from a study that utilized an in vivo model to assess the antibody-dependent cellular cytotoxicity (ADCC) activity of antibodies to clear HIV-infected cells.

• Antigen-Specific Restimulation Of CD8+ T Cells By Cross-Presenting DCs

  High-quality automated cell isolation and consistent cell culture and stimulation conditions are required for successful immuno-oncology studies. See how CD8+ T cells and XP-DCs were isolated using MACS® technology.

• Automated Isolation Of CD56 NK Cells And Antibody-Dependent Cellular Cytotoxicity

  Explore results from using both flow cytometry and the LDH assay for the isolation of NK cells and an ADCC assay with the MultiMACS™ Cell24 Separator Plus.

• A Complete Macrophage Suppression Assay Workflow

  Analyze an experiment conducted to validate a macrophage suppression assay workflow and demonstrate a consistent in vitro macrophage suppression assay used in the validation process.

• Mo-DC Generation: From Monocyte Isolation To DC Characterization

  Explore the steps involved and the products used in the generation of functional monocyte-derived DCs (Mo-DCs) that are suitable for a variety of research and immunotherapy applications.

• Reliable, Hands-Free, And High-Throughput Cell Viability Assays

  To validate the importance of using an automated PI staining process with a MACSQuant® Flow Cytometer, we processed various 96-well plates of Jurkat cells under different conditions.

• Functional TILs From Human Renal Cell Carcinoma

  Here, we compare two methods for the dissociation of renal tumor tissue to obtain TILs: a novel, combined mechanical/enzymatic, automated 3-hour process versus an established overnight digestion protocol.

• Gentle Sorting Of PBMC

  Successful cell sorting relies on the collection of viable cells, and new technology is being designed to address cell sorting concerns, such as cell damage and sterility.

• Easy And Effective Isolation Of CD8+ Antigen-Specific T Cells

  Here, we demonstrate the ability of a fully closed, sterile cell sorter to sort rare antigen-specific cytotoxic T cells out of fresh peripheral blood mononuclear cells (PBMCs).

• Isolation Of TILs Improves The Sensitivity Of Single-Cell Immune Profiling

  Look further into the study of a new workflow for the isolation and analysis of tumor-infiltrating lymphocytes to see how it could become a valuable tool for immunotherapy researchers.

• GMP-Compliant Flow Cytometric Cell Sorting Of Antigen-Specific T Cells

  Here, we demonstrate the generation of large numbers of CD8+CD137+ antigen-specific T cells with high purity based on GMP-compatible reagents and sorting system.

• Flow Cytometric Assays For CAR T Cell Manufacturing And Patient Monitoring

  Unprecedented efficacy in targeting cancer has been demonstrated with CAR T cell therapy. Discover a device that allows the generation of CAR T cells in a single automated and closed system.