Multicolor Immunophenotyping Of Human Tumor Cells Using MACSQuant® Analyzer 16

By Manuela Herber, Philipp Gert, Cesar Evaristo, Elvira Criado-Moronati, Anne Richter, and Christian Dose

Flow cytometric phenotyping is an invaluable technique utilized across a broad spectrum of applications, ranging from fundamental research to diagnostics and clinical monitoring. This method is particularly crucial in the rapidly advancing field of immunotherapy, where understanding the nuances of steady-state immunity and the impacts of therapeutic interventions on the immune system is paramount. One of the most promising strategies in immunotherapy involves harnessing T cells to target and destroy tumors, which has demonstrated efficacy in treating various types of cancer. However, it is important to note that these therapeutic approaches often benefit only a subset of patients.

To achieve accurate immune monitoring, it is vital to employ standardized reagents and procedures that minimize inter- and intra-assay variability. This is especially important when sample availability is limited, as maximizing the information obtained from flow cytometric analysis becomes crucial. In this context, we present the standardization of flow cytometry assays through the use of a ready-to-use 8-color immunophenotyping cocktail designed for whole blood sample analysis.

Discover how this advanced approach allows for a deeper understanding of the immune landscape within tumors and helps facilitate more effective and personalized immunotherapy strategies.