Multiparameter Characterization Of CAR-T Cells

By Xueting Wang, Christina Pitzka, Daniela Rheindorf, Julia Milleck, Nadine Mockel-Tenbrinck, Dr. Tatjana Holzer, Dr. Mike Essl, Dr. Iris Bürger, Dr. Marsilius Mues, Dr. Anne Richter, Prof. Dr. Toni Cathomen, and Dr. César Evaristo

Adoptive cell transfer involving chimeric antigen receptor (CAR) -modified T cells has shown remarkable therapeutic success in combating certain types of cancer. Despite these advancements, the effectiveness of CAR-T cell treatments can vary significantly due to mechanisms that remain partially understood and difficult to control. As research progresses, numerous innovative CAR-T cell-based therapies are being developed to enhance treatment outcomes.

A critical aspect of advancing these therapies is the thorough characterization of CAR-T cells during all stages of pre-clinical and clinical development. Reliable tools and methods are essential for this characterization to ensure the safety, efficacy, and consistency of CAR-T cell products. To address this need, we have established a comprehensive set of in vitro assays designed for the multiparameter characterization of CAR-T cell products. These assays provide detailed insights into the properties and behaviors of CAR-T cells, facilitating their application throughout all phases of drug development.

Discover how employing these assays can help researchers better understand the complex dynamics of CAR-T cell therapies and optimize their development for clinical use.