Isolation Of Antigen Specific CD8+ T cells Using A Closed, Sterile, Single-Use Microchip-Based Cell Sorter

With Adoptive T Cell Therapies (ACTs) for cancer becoming more prevalent, the need for isolation and expansion of cells in a clinical setting is growing. The MACSQuant® Tyto™ is a bench top sorter that is fully closed, sterile, and easy to use. The heart of the system is in the disposable, single use, cartridge. This cartridge allows for completely aseptic sorting conditions with no chance of cross contamination between samples or patients. Here, we demonstrate this capacity by using the MACSQuant® Tyto™ to sort cancer antigen specific cytotoxic T cells out of stimulated peripheral blood mononuclear cells (PBMCs).