Application Note

StemMACS™ HSC-CFU Assay Kit, Human: Flow Cytometric Analysis Of The Differentiation Potential Of Human Hematopoietic Stem Cells

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The StemMACS HSC-CFU Assay Kit, human, offers an exceptional blend of a methylcellulose-free medium that simplifies handling and a clear flow cytometric readout, which eliminates the need for subjective visual scoring under a microscope. Utilizing the MACSQuant® Analyzer X alongside a plate-feeding incubator enhances automation, reduces manual intervention, and facilitates high-throughput sample acquisition.

In this application note, human buffy coat-derived hematopoietic stem and progenitor cells (HSPCs) were analyzed using the StemMACS HSC-CFU Assay Kit, human. This kit and the described procedure are also suitable for analyzing HSPCs from other human sources, such as peripheral blood, cord blood, bone marrow, or mobilized leukapheresis.

Explore how this versatility makes the kit a valuable tool for various research and clinical applications.

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