FEATURED ARTICLES

• High Sequence Coverage And Confident PTM Analysis Of Biotherapeutics Using An EAD-Based Workflow

  Explore how a streamlined, EAD-based middle-down workflow can achieve consistent high sequence coverages of mAb subunits and confident PTM analysis in a single injection.

• Monitoring And Determining The Cause Of Antibody Discoloration Using cIEF And EAD

  View a study which provides additional insight into the factors that govern the discoloration of protein therapeutics and the role of AGEs in color change.

• EAD-Based Middle-Down Workflow Analysis

  How can a single-injection, EAD-based middle-down workflow leverage accurate mass measurement, information-rich EAD fragmentation and automated data analysis to offer confident sequence confirmation.

• CE-SDS Analysis Of Etanercept Using The BioPhase 8800 System

  In this study, a system with multi-capillary capabilities was used to analyze etanercept by bapillary electrophoresis-sodium dodecyl sulfate (CE-SDS).

• mRNA-LNP Nucleic Acid Assessment From Distinct Formulations

  Review a showcase of a high throughput robust analysis for nucleic acid integrity and size assessment of mRNA-LNP samples using a multi-capillary system and purity and integrity analysis kit.

• Molecular Weight Confirmation For Length, Integrity And Impurity Analysis Of A 70-Mer Oligonucleotide

  Explore a workflow used to determine the molecular weight and analyze the purity of a 70-mer DNA sequence with a 3’ dT overhang.

• Purity Analysis Methods For Synthetic Prime Editing Guide RNAs

  Develop a fast, easy workflow for the purity analysis of complex pegRNA molecules along with a robust denaturing technique for pegRNA with high levels of secondary structure.

• Enabling Digital Innovation To Transform Cell Line Development

  Establishing an integrated, tailored data management strategy is core to getting the cell line development journey right.

• Structural Characterization Of The Cationic LNP Component

  Gain a deeper understanding of how EAD is used when characterizing ALC-0315 and its impurities, as well as identify the nature and site of chemical alterations in ALC-0315 resulting from forced degradation.

• Characterization And Relative Quantification Of mRNA 5’-Capping

  Review the development of an LC-MS method for the reliable identification and relative quantification of different mRNA capping structures.

• Distinguishing Oxidative Impurities From Ionizable Lipids Used In LNP Formulations

  A test conducts on a novel fragmentation mode for the detailed characterization of lipids, reveals information that can be used to determine drug efficacy and safety from formulated LNPs.

• Comprehensive Peptide Mapping Of Protein Therapeutics

  Explore how the peptide mapping workflows templates provided by a software designed for the characterization of biotherapeutic digests alongside data visualization and interrogation.

• Transforming Analytics To Optimize Cell Line Development

  For cell line development, much of a workflow’s success hinges on how much analytical data a team can get – and how quickly – to inform their process.

• LCMS/MS Methodology For Mapping Disulfide Linkages In Etanercept

  Learn about the details surrounding the comprehensive mapping of disulfide linkages in etanercept using the EAD approach on an analytical characterization system.

• Characterize Deglycosylated NISTmAb Using icIEF-UV/MS

  Examine a comprehensive analysis of deglycosylated NISTmAb (dgNISTmAb) using a novel integrated icIEF-UV/MS workflow.

• Direct And Rapid Multi-Attribute Monitoring Of Multiple Intact mAbs

  What are the results of an innovative system for direct chip-based integration of icIEF with mass spectrometry (MS) being utilized to characterize mAbs spanning a wide pl range?

• Peptide Mapping Study On Human Erythropoietin

  A newly integrated and easy-to-use peptide mapping workflow for the analysis of standard monoclonal antibodies provides a streamlined workflow for the analysis of both CID and EAD data.

• Electron Activated Dissociation Workflow For Biotherapeutics Characterization

  Explore the benefits of an electron-activated dissociation middle-down workflow, including the ability to achieve high sequence coverages (70%-80%) of monoclonal antibody subunits in a single injection.

• Combining Single-Cell Isolation And Microplate Imaging To Improve Cloning Efficiency

  Improve your cloning efficiency with higher assurance of clonality by using a combination of single-cell isolation and microplate imaging.

• Automation Of The CRISPR- Based Cell Line Development Workflow

  Review an in-depth study of the automation of a mammalian cell-line development workflow—the automated, integrated screening of transfected cells for edits, monoclonality, and growth assessment.