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SpectraMax iD3s And iD5e Multi-Mode Microplate Readers

Scientist standing with SpectraMax iD5e mutlt-mode microplate reader gas mixer included

Designed for today’s assays and tomorrow’s breakthroughs—flexible detection that evolves with your science.

The SpectraMax® iD3s and iD5e Multi-Mode Microplate Readers deliver flexible, scalable solutions for absorbance, fluorescence, and luminescence assays—with advanced capabilities for evolving research needs. The iD3s reader offers a streamlined, affordable platform with a tunable monochromator and temperature control, while the iD5e reader adds enhanced detection modes including time-resolved fluorescence (TRF) and tunable fluorescence polarization (FP) and can be expanded to include TR-FRET, HTRF, BRET and western blot. Both readers support on board fluidics with optional dual injectors.

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For live-cell assays and microbial growth experiments, environmental control for CO₂/O₂ regulation and advanced shaking are available to maintain ideal growth conditions. Data acquisition and analysis are driven by SoftMax® Pro Software—the most cited microplate reader software in the industry.

Supports diverse assay needs

  • Perform ELISA, nucleic acid and protein quantitation, cell viability, microbial assays, western blot, and more.

Simplified setup & analysis

  • Over 200 preconfigured protocols and intuitive setup tools help streamline data acquisition and analysis—backed by the industry’s most cited microplate reader software.

Fast, consistent mixing for high-performance flash assays

  • Dual injectors available for fast kinetic assays with SmartInject® Technology, ensuring complete reagent mixing in every well for high-precision with 10 µL dead volume.

Features

Superior hybrid optical system

  • The high-efficiency monochromator and filter-based optical system, combined with an ultra-cooled photomultiplier tube (PMT), minimizes noise while increasing sensitivity, selectivity, and dynamic range.

Live cell assays

  • Advanced shaking and user-installable gas mixer (CO2/O2) that support ideal environments for cell-based and microbial growth experiments are available as optional upgrades.

Automatic filter identification

  • Automatically identify filter sets using NFC functionality, simplifying workflows and ensuring accuracy and reliability.

Flexible temperature control

  • Precise and intuitive temperature regulation—from 5°C above ambient conditions up to 66 °C—empowering your lab to perform a wide range of temperature-sensitive assays with confidence.

Read height optimization (Z height)

  • Z-height optimization automatically adjusts the detection height to maximize sensitivity and signal quality—while minimizing sample volume—ensuring reliable results even in low-volume or precious samples.

Microvolume & cuvette options

  • The SpectraDrop Micro-Volume Microplate for low-volume (2 to 4 μL) and SpectraCuvette™ Adapter Plate for cuvette-based applications eliminate the need for additional instrumentation.

Personalized touchscreen control

  • The intuitive touchscreen software simplifies tasks with Quick Read icons that save time when starting new experiments and a personalized library that stores frequently used protocols.

Western blot capable

  • The optional enhanced time-resolved fluorescence (TRF) detection module is optimal for quantitating as little as femtogram protein samples, providing improved sensitivity, extended signal stability and versatility to support a wide range of applications.

Danaher Life Sciences