Extraction Of Oligo Therapeutics From Biological Samples For LC-MS Bioanalysis

This asset presents a solution designed to streamline the extraction of oligo therapeutics from biological samples for LC-MS bioanalysis, addressing challenges like low recovery and MS ion suppression. The Clarity OTX™ extraction solution utilizes mixed-mode Solid Phase Extraction (SPE) with carefully formulated buffers to achieve high recoveries, consistently delivering greater than 80%. The protocol is a simple, four-step process that can be completed in approximately 15 minutes, eliminating the time and labor-intensive Liquid-Liquid Extraction (LLE) step found in common procedures.

The method supports high-throughput analysis and is amenable to liquid handling automation via a 96-well plate format. Results demonstrate excellent linearity across a broad dynamic range and high selectivity, effectively removing plasma contaminants and minimizing background noise to enhance LC-MS sensitivity. The solution can be used to extract a variety of oligonucleotide classes from matrices like plasma, urine, serum, and tissue homogenate. Access the full details to see how this simplified workflow can transform your bioanalytical process.