Featured Articles

81. Efficient Expansion of Suspension CHO Cells in Corning® 5 Liter Erlenmeyer Flasks 8/4/2021

    Erlenmeyer flasks are commonly used for expansion of a variety of suspension cell lines for bioprocessing applications. In this study, we describe a protocol to scale up suspension CHO cells from 40 mL to 15L of total volume in just 12 days using Corning 5L Erlenmeyer flasks. Furthermore, we demonstrate that Corning 5L Erlenmeyer flasks provide a more favorable culture environment (higher oxygenation and lower lactate metabolite accumulation) and support a higher yield of viable CHO cells when compared to competitor 5L Erlenmeyer flasks.

82. The Corning® 5L Erlenmeyer Flask Provides Efficient Scale-Up Of Suspension Cells 8/4/2021

    As the bioprocess industry grows, there is a corresponding growth in demand for single-use containers that can efficiently culture large quantities of cells. To aid in this effort, Corning has developed a 5L Erlenmeyer flask featuring a design that allows for the culture volume to be increased to greater than one half of the stated volume of the vessel without compromising cell viability or density. In this study, we demonstrate that the Corning 5L Erlenmeyer flask exhibits enhanced performance when culturing a suspension cell line commonly used for baculovirus production.

83. Choosing Optimal Harvesting Solutions 5/24/2021

    Although some harvesting reagents are more common than others, each can impact cell recovery, viability, and functionality. Here, we highlight commonly used harvesting reagents and their advantages.

84. Corning® X-LAB® System For Closed, Sterile PBMC Recovery 5/24/2021

    Research and therapies involving the human immune system rely heavily on peripheral blood mononuclear cells (PBMCs) isolated from whole blood sources. The Corning X-LAB system is designed to isolate and harvest PBMCs from blood and blood products in a closed, sterile device that is automation-friendly. Here we show how the Corning X-LAB system can deplete more than 99% of red blood cells and 80% of platelets.

85. Characterization And Analysis Of Dissolvable Microcarrier Byproducts 5/24/2021

    Microcarriers enable efficient cell scale-up in controlled bioreactors. Effective cell recovery from microcarriers often requires the use of concentrated enzymes, extended treatment times, and continuous centrifugation/filtration cycles, which can negatively affect cell health and recovery yield. To address these challenges, Corning Life Sciences developed a calcium-crosslinked polygalacturonic acid (PGA) microcarrier that can be dissolved using EDTA and pectinase. Here we describe the characterization of the PGA polymer, dissociation reagents, and the byproducts of bead dissolution and evaluate their impact on subsequent cell growth.

86. Maximizing Yield For Attachment-dependent Cells With The Corning® CellCube® System 5/24/2021

    As cell therapy trials advance from pre-clinical research to late-stage trials, cell culture platforms will need to meet increasing demands for cell yield. Establishing an adherent culture platform that can scale from relatively small needs of pre-clinical studies through Phase I/II trials and into Phase III will be a challenge. In this study, the CellCube system was used for expansion of two commonly used bioproduction cell lines – HEK293T and Vero - resulting in final harvest yields for both HEK293T and Vero cell lines greater than 10 billion cells.

87. What's Killing My Cell Cultures? Troubleshooting Cell Growth Issues 5/14/2021

    Stunted cell growth and erratic culture behavior can delay projects, frustrate teams, and leave researchers wondering what went wrong and how to can fix it. 

88. Working With Viral Vectors: New Trends To Optimize Your Workflow 5/14/2021

    Scientists had been increasingly using viral vectors in therapeutic research well before the coronavirus pandemic, as their use in gene therapy and cell therapy has proven promising. Now, those same applications are being repurposed to develop a coronavirus vaccine. However promising they might be, viral vector workflows come with inherent challenges in scale and process. Fortunately, technology is evolving rapidly, and new solutions to simplify viral vector bioprocessing are continuing to emerge.

89. Vaccine Production Process Development: 4 Best Practices For Scaling Up 5/14/2021

    The vaccine production process is continually refreshed by new tools and technologies which imposes challenges on process development scientists: They're the ones who have to align and optimize new methods and means with legacy products and systems. By carefully crafting a customized modular process from end to end, process development scientists can glean the benefits of new technologies without starting from scratch with each new iteration. How can researchers working on vaccine production optimize their process with customization in mind?

90. 5 Things To Consider When Customizing Cell Culture Media 5/14/2021

    Are you searching for a customized solution for your media, sera, or reagents? Here's what you should consider as you contemplate your options for bespoke lab products.