Featured Articles

71. Apply The Right Hydrogel In Your Microplates For Better 3D Cell Culture Research 12/19/2021

    With emerging innovations in bioprinting and adaptive bioengineering, the innovations just keep on growing, flooding the market with exciting new options to add to your lab's 3D cell culture toolkit. But which type of hydrogel is right for you?

72. 3D Cell Culture Applications And Bioprinting Will Change How We Research Cancers 12/19/2021

    Complex aspects of cancer like metastasis can be modeled and studied, and the efficacy and toxicity of drugs can be tested more realistically and rapidly with the advent of 3D cell culture applications and 3D bioprinting. Learn how 3D bioprinting works and how it is advancing cancer research.

73. How 3D Cultures Better Mimic In Vivo Conditions In The Field 12/16/2021

    Although the traditional 2D cell culture is still used as a primary experimental tool all across the world, we look into how 3D cultures better mimic in vivo conditions because they provide cells the physical environment they need to interact naturally.

74. In Vivo-like Cell Culture: The Impact Of 3-Dimensional Technologies On Your Cell-based Applications 12/14/2021

    The limitations of 2D cell culture can cause roadblocks to research and contribute to poor predictive power of preclinical cell-based drug and toxicity screening assays. In this article we explore several more reasons why 3D is better and provide tips for perfecting the technique for optimal results.

75. Bone Marrow-Derived Human Mesenchymal Stem Cell Production In HYPERStack® 36-layer Cell Culture Vessels 8/18/2021

    Although Mesenchymal stem cells (MSCs) can be isolated from different tissue sources, bone marrow-derived MSCs are commonly studied due to their ease of access and achievable therapeutic dosage (2 x 10⁶ cells/kb of body weight). Here, we demonstrate the utility of the Corning HYPERStack 36-layer cell culture vessel as a tool to meet the growing demand for expanding bone marrow-derived MSCs to relevant scale for clinical application workflows.

76. Transfection Best Practices For AAV Gene Therapy Programs 8/17/2021

    As viral vectors continue to push gene therapy innovations closer to market, many researchers are setting their sights on optimizing the process of transfection — that is, the process of delivering corrective genetic material into cells. It's not just a question of how to transfect, but also how to do it efficiently and at a high volume. Approaches that may work for one cell line may not work for another, and all transfection protocols can have varying implications for scalability and cost during production for clinical trials. This article explores some best practices for transfection.

77. Is A Perfusion Cell Culture System Right For Adherent Cultures? 8/17/2021

    How do you know when to make the switch to a perfusion setup like the Corning® CellCube® System? We asked our industry experts what scientists need to know.

78. High-Level Transient Recombinant Protein Production 8/4/2021

    In this study, we describe a method for the transient transfection of CHO cells in Corning 5L Erlenmeyer flasks, which allow for enhanced recombinant protein production.

79. Efficient Expansion of Suspension CHO Cells in Corning® 5 Liter Erlenmeyer Flasks 8/4/2021

    Erlenmeyer flasks are commonly used for expansion of a variety of suspension cell lines for bioprocessing applications. In this study, we describe a protocol to scale up suspension CHO cells from 40 mL to 15L of total volume in just 12 days using Corning 5L Erlenmeyer flasks. Furthermore, we demonstrate that Corning 5L Erlenmeyer flasks provide a more favorable culture environment (higher oxygenation and lower lactate metabolite accumulation) and support a higher yield of viable CHO cells when compared to competitor 5L Erlenmeyer flasks.

80. The Corning® 5L Erlenmeyer Flask Provides Efficient Scale-Up Of Suspension Cells 8/4/2021

    As the bioprocess industry grows, there is a corresponding growth in demand for single-use containers that can efficiently culture large quantities of cells. To aid in this effort, Corning has developed a 5L Erlenmeyer flask featuring a design that allows for the culture volume to be increased to greater than one half of the stated volume of the vessel without compromising cell viability or density. In this study, we demonstrate that the Corning 5L Erlenmeyer flask exhibits enhanced performance when culturing a suspension cell line commonly used for baculovirus production.