Featured Articles

91. Improving Scale-Up Efficiency 4/27/2021

    A compilation of research experiences using an industry standard for a variety of adherent cell expansion applications, including viral vector manufacturing, stem cell expansion, and vaccine production.

92. Corning® KBM581: Nutritious, Serum-free Media For CAR-T Cell Expansion 4/26/2021

    When considering an adoptive cell therapy approach, the transfusion of a sufficient number of CAR-T cells with an appropriate phenotype is critical. Corning KBM581 has been shown to be a nutritious, safe, and serum-free medium for other immune cell therapies, such as CIK and NK cells. To discover which medium among several commercialized media (including KBM581) is most appropriate for supporting CAR-T cell growth, the research group from Bioraid Bio generated CAR-T cells and analyzed the expansion folds and phenotype between different media in this application note.

93. CRISPR/Cas9-mediated PD-1 Knock-out In Human Primary T Cells Shows Enhanced Cytotoxicity Against Cancer Cells 4/26/2021

    The existence of an adaptive immune system in single-cell organisms was debated for a long time until the CRISPR/Cas9 mechanism had been identified. In this study, the CRISPR/Cas9 system was used to knock-out PD-1 gene expression in human primary T cells.

94. Peripheral Blood Mononuclear Cell Isolation 4/26/2021

    Peripheral blood mononuclear cells (PBMCs) are valuable for both clinical and research applications. Isolating pure populations of PBMCs from whole blood traditionally requires sample dilutionand use of a density gradient medium to deplete red blood cells (RBC), granulocytes (GRN) and platelets (PLT). This open, manual process involves a high risk of contamination and is made up of multiple tedious steps.  The Corning X-LAB System is a functionally closed, sedimentation-based system that reliably and reproducibly isolates PBMCs without the need for density gradient media or manual transfer steps.

95. Counting Peripheral Blood Mononuclear Cells Using The Corning® Cell Counter 4/26/2021

    Peripheral Blood Mononuclear Cells (PMBCs) are often counted using time-consuming manual methods such as a hemocytometer. While the hemocytometer has been considered the gold standard for cell counting since the 18th century, results are frequently subjective, variable, and small differences in cell diameters cannot easily or reproducibly be discerned. The Corning Cell Counter offers an alternative method to quickly and accurately count these cells.

96. Corning® 100 mm Aseptic Transfer Cap For 5L Erlenmeyer Flasks: An Aseptic Solution For Liquid Transfer 4/26/2021

    Closed cell culture systems play a significant role in reducing the risk of contamination during liquid handling. The Corning 100 mm aseptic transfer cap (ATC) offers a closed system solution for transferring liquid aseptically into or out of a Corning 5L Erlenmeyer flask. Here we demonstrate the use of the ATC to seed and expand two suspension cell lines, 5/9 m alpha3-18 and Sf9 cells in the 5L Erlenmeyer flasks.

97. Counting Murine CAR T Cells Using The Corning® Cell Counter 4/26/2021

    CAR T cells are often counted using time consuming manual methods such as a hemocytometer. While the hemocytometer has been considered the gold standard for cell counting since the 18th century, results are frequently subjective, variable, and small differences in cell diameters cannot easily or reproducibly be discerned. This application note demonstrates the Corning Cell Counter offering an alternative method to quickly and accurately count these cells.

98. Efficient Expansion Of HEK-293 Suspension Cell Cultures 4/26/2021

    In this study, we assessed the performance of Corning 5L Erlenmeyer flasks with different media fill volumes on the growth of HEK-293F cells, a variant of the HEK-293 cell line

99. Effective Bead-to-Bead Transfer Of Cells In Serum-free Medium Using Polystyrene Corning® Enhanced Attachment Microcarriers 4/26/2021

    To achieve large-scale cell production, a microcarrier culture scale-up strategy is necessary. Several approaches have been developed to achieve the transfer of cells from one microcarrier to another including enzymatic dissociation and bead-to-bead transfer. However, enzymatic dissociation introduces additional process steps (washing, inactivation, and removal of the enzyme) that are not feasible at a large scale. This report describes a method for expansion of anchorage-dependent cells on polystyrene Corning microcarriers with an enhanced attachment surface treatment in serum-free cell culture medium.

100. Efficient Expansion Of Suspension CHO Cells In Corning® PETG Erlenmeyer Flasks 4/26/2021

     The adoption of single-use technologies for biopharmaceutical manufacturing is a rapidly growing trend due to a number of advantages including the reduced risk of contamination, reduced capital costs, and faster time to market. In this study, we assessed the growth of CHO-S cells in Corning 2L, 3L, and 5L PETG flasks with different fill volumes using a typical batch culture protocol. Our results demonstrate efficient expansion and high viability of CHO-S cells in all three flask sizes during 6 days in culture.