Featured Articles

91. Hospital Researchers Turn To Corning To Help Scale Up Ex Vivo Expansion Of Adipose Stem Cells 5/13/2021

    Autologous fat grafting is the least invasive method for breast reconstruction, but also has major drawbacks, including high tissue resorption rates of up to 80%. Researchers in Denmark had a hypothesis: what if you could enrich the human fat graft with autologous adipose stem cells (ASCs) to increase graft survival? But they faced a major obstacle: how do you culture a sufficient volume of stem cells to increase grafting success? They turned to Corning, who designed a scale-up solution that’s enabling them to grow the billions of stem cells required to move forward with this clinical study.

92. First Clinical Trial Using Stem Cell Therapy To Treat Septic Shock 5/12/2021

     An Ottawa Hospital Research Institute’s Cell Manufacturing Facility successfully conducted a Phase I clinical trial examining the safety and efficacy of allogeneic bone marrow-derived MSCs as a treatment for patients experiencing septic shock. Before initiating clinical Phase II studies, the protocol for generating large quantities of MSCs had to be established to reduce end-product variability, minimize production costs, and to ensure sufficient yield to treat multiple patients. Maximizing the yield of viable cells proved challenging in a limited footprint.

93. Process Development In Gene Therapy: 4 Things To Know 5/12/2021

    Considering everything it takes to scale up gene therapy — the platforms, the media, the surfaces, the vessels, the equipment — developing viral vector production processes comes with many challenges.

94. Improving Scale-Up Efficiency 4/27/2021

    A compilation of research experiences using an industry standard for a variety of adherent cell expansion applications, including viral vector manufacturing, stem cell expansion, and vaccine production.

95. Corning® KBM581: Nutritious, Serum-free Media For CAR-T Cell Expansion 4/26/2021

    When considering an adoptive cell therapy approach, the transfusion of a sufficient number of CAR-T cells with an appropriate phenotype is critical. Corning KBM581 has been shown to be a nutritious, safe, and serum-free medium for other immune cell therapies, such as CIK and NK cells. To discover which medium among several commercialized media (including KBM581) is most appropriate for supporting CAR-T cell growth, the research group from Bioraid Bio generated CAR-T cells and analyzed the expansion folds and phenotype between different media in this application note.

96. CRISPR/Cas9-mediated PD-1 Knock-out In Human Primary T Cells Shows Enhanced Cytotoxicity Against Cancer Cells 4/26/2021

    The existence of an adaptive immune system in single-cell organisms was debated for a long time until the CRISPR/Cas9 mechanism had been identified. In this study, the CRISPR/Cas9 system was used to knock-out PD-1 gene expression in human primary T cells.

97. Peripheral Blood Mononuclear Cell Isolation 4/26/2021

    Peripheral blood mononuclear cells (PBMCs) are valuable for both clinical and research applications. Isolating pure populations of PBMCs from whole blood traditionally requires sample dilutionand use of a density gradient medium to deplete red blood cells (RBC), granulocytes (GRN) and platelets (PLT). This open, manual process involves a high risk of contamination and is made up of multiple tedious steps.  The Corning X-LAB System is a functionally closed, sedimentation-based system that reliably and reproducibly isolates PBMCs without the need for density gradient media or manual transfer steps.

98. Counting Peripheral Blood Mononuclear Cells Using The Corning® Cell Counter 4/26/2021

    Peripheral Blood Mononuclear Cells (PMBCs) are often counted using time-consuming manual methods such as a hemocytometer. While the hemocytometer has been considered the gold standard for cell counting since the 18th century, results are frequently subjective, variable, and small differences in cell diameters cannot easily or reproducibly be discerned. The Corning Cell Counter offers an alternative method to quickly and accurately count these cells.

99. Corning® 100 mm Aseptic Transfer Cap For 5L Erlenmeyer Flasks: An Aseptic Solution For Liquid Transfer 4/26/2021

    Closed cell culture systems play a significant role in reducing the risk of contamination during liquid handling. The Corning 100 mm aseptic transfer cap (ATC) offers a closed system solution for transferring liquid aseptically into or out of a Corning 5L Erlenmeyer flask. Here we demonstrate the use of the ATC to seed and expand two suspension cell lines, 5/9 m alpha3-18 and Sf9 cells in the 5L Erlenmeyer flasks.

100. Counting Murine CAR T Cells Using The Corning® Cell Counter 4/26/2021

     CAR T cells are often counted using time consuming manual methods such as a hemocytometer. While the hemocytometer has been considered the gold standard for cell counting since the 18th century, results are frequently subjective, variable, and small differences in cell diameters cannot easily or reproducibly be discerned. This application note demonstrates the Corning Cell Counter offering an alternative method to quickly and accurately count these cells.