Corning® 100 mm Aseptic Transfer Cap For 5L Erlenmeyer Flasks: An Aseptic Solution For Liquid Transfer

By Hilary A. Sherman, Lori Romeo, and Hannah Gitschier, Corning Incorporated, Life Sciences

Closed cell culture systems play a significant role in reducing the risk of contamination during liquid handling. The Corning 100 mm aseptic transfer cap (ATC) offers a closed system solution for transferring liquid aseptically into or out of a Corning 5L Erlenmeyer flask. The ATC also contains a hydrophobic vent with 0.2 μm pore size for gas exchange, making the cap ideal for use during cell culture. The flexible tubing has a male medical plastic coupler (MPC) and is also heat sealable, allowing for a multitude of connectivity options. The vessel can be filled via gravity flow, peristaltic pump, or with negative pressure applied to the 0.2 μm vent filter of the ATC. To empty the vessel, a peristaltic pump can be used, or positive pressure can be applied to the transfer cap vent filter. Here we demonstrate the use of the ATC to seed and expand two suspension cell lines, 5/9 m alpha3-18 and Sf9 cells in the 5L Erlenmeyer flasks.