Thermo Fisher Scientific Bioproduction Articles
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Human Regulatory T Cell Expansion And Measurement Of Function
8/17/2021
Regulatory T cells (Tregs) are a subpopulation of T cells with the unique ability to suppress the activation of effector T cells and thereby prevent T cell function. This application note describes an optimized protocol for activation and expansion of flow-sorted Tregs with Gibco™ CTS™ Dynabeads™ Treg Xpander.
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Bacto CD Supreme FPM Consistently Supports Higher Growth And Plasmid Production In E. coli
8/12/2021
Traditional microbial media for E. coli often contain animal-origin components and are complex. Stemmed from an overall industry drive to develop animal origin–free (AOF) and chemically defined (CD) media, Gibco™ Bacto™ CD Supreme Fermentation Production Medium (FPM) supports process flexibility and consistency in production. Read the results of a head-to-head comparison between Bacto CD Supreme FPM and Terrific Broth (TB), a traditional microbial medium.
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One-Step Isolation And Activation Of Naive And Early Memory T Cells With CTS Dynabeads CD3/CD28
7/6/2021
In this application note we demonstrate nearly 100% recovery of naive and early memory T cells using Gibco™ CTS™ Dynabeads™ CD3/CD28 beads. This one-step process of simultaneous T cell isolation and activation yields pure and uniformly activated T cells.
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Understanding Perfusion Terminology
6/7/2021
To evaluate whether a perfusion application will work in a production process, standardized terminology is critical. This white paper explains key terms that are essential for understanding the process.
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Outsourcing Trusted-Weight Dry Powder Chemicals To Support Expansion
2/5/2021
The gene therapy division of a major pharmaceutical manufacturer was looking for ways to overcome capacity constraints due to the increased production demands for process liquids and buffers. Non-core manufacturing activities, including the weigh and dispense steps in the process liquid and buffer preparation, were identified as bottlenecks impacting the overall production schedule. The proposed solution would provide ready-to-hydrate, trustedweight dry powder chemicals. Read how the customer benefited from the procurement of larger lot sizes that were not capable of being handled within their facility, resulting in annual savings for raw materials and QC testing.
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Culture Of Human Mesenchymal Stem Cells In Nunc Cell Factory Systems
4/12/2018
Helpful methodology that can be applied to the large-scale expansion of other cell types for use in various applications using Cell Factory systems.