Bio-Techne Application Notes
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Automated, Rapid, Reproducible Quantification Of HIV-1 Gag p24
2/22/2021
Reliable methods for quantification of Lentiviral vectors support process development and enables quality control of both vector and cell manufacturing. Traditionally ELISA assays serve as a common methodology for calculating viral titres and are used to measure the amount of viral capsid p24 protein in the supernatant of packaging cell lines. The 3.5 to 6 hours required to run a Standard ELISA is a challenge for operators who who increasingly require results quickly to inform real time decision making. The Simple Plex p24 assay, hosted on Ella - an automated immunoassay system, can rapidly quantify p24 levels expressed from lentiviral vectors during different stages of cell and vector manufacturing.
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A Fixation Protocol Compatible With R&D Systems Luminex Assays Enabling Analysis With Infectious Samples
2/22/2021
A common way to inactivate infectious material is to use formaldehyde fixation. Confirmation of microorganism inactivation is accomplished using viability testing with broth or growth media for bacteria and plaque assays for viruses. Unfortunately, chemical fixation using formaldehyde has been shown to alter cytokines and chemokines in such a way that an immunoassay would not properly quantify biological samples. To address this issue, we decided to test the hypothesis that R&D Systems Luminex Assays are compatible with standard fixation protocols.
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Residual Protein A For Bioprocessing Applications
2/19/2021
Most therapeutic monoclonal antibodies (mAbs) are isolated from cell culture media using Protein A chromatography. During purification, Protein A can leach from the solid phase, ending up in the purified product. This can be problematic as regulatory guidelines dictate that detection and reduction of residual Protein A are necessary to obviate potential immunogenic consequences in vivo. ProteinSimple’s Wes™ provides a powerful solution by bringing immunoassay into a capillary electrophoresis format.
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Automated Particle Analysis Of Viscous Samples With MFI And The Bot1 Autosampler
2/19/2021
Micro-Flow Imaging™ (MFI) easily detects particle size and morphology on a wide range of particle contaminants. And when you add the Bot 1 Autosampler to your MFI 5000 Series system, you’ve got the automated go-to method of choice for particle analysis today. In this application note, we studied how the Bot1 handled viscous samples, and found that with just a few simple optimization steps, you can be running viscous samples in no time at all.
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Protein Expression Heterogeneity With Milo, The First Single-Cell Western System
2/18/2021
Every cell is unique, making cell-to-cell heterogeneity important in many areas of biomedicine including cancer pathogenesis, immuno-oncology and regenerative medicine. More and more high-profile publications are using single-cell analysis techniques to reveal variability in cellular response to a drug or stimulus. Milo, the first and only Single-Cell Western platform out there, lets you do Western blotting at the single-cell level.
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A Custom, Multiplex Human Coronavirus Serological Assay
2/18/2021
R&D Systems, a Bio-Techne brand, has developed a novel custom Luminex serological assay hat distinguishes between coronavirus subtypes. Using our high-quality recombinant proteins, this antigen-down assay saves time and precious sample by multiplexing many coronavirus subtypes simultaneously in each sample.
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Wes And Milo Synergize To Profile Immune Cell Populations In The Tumor Microenvironment
2/4/2021
The population of patients who respond to immunotherapy are a minority. Accumulating evidence suggests that the heterogeneous makeup of the tumor microenvironment (TME) is a major contributor to the discrepancies in response observed. Profiling the composition and function of immune cells that exist within the TME is poised to guide an improved response to immunotherapy and may uncover novel therapeutic targets and strategies. In this application note, we’ll show you how Wes™ and Milo™ partner to get you critical answers to what type of immune cell populations are present in a sample and then what percentage of cells in that sample make up a specific immune cell subtype.
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Reprobe Your Immunoassay Samples Using RePlex With Jess
2/4/2021
The identification and quantification of protein expression levels is critical to understanding biological processes like those that drive cancer and other diseases. Unfortunately, limited samples sizes are far too common in these research areas, leaving researchers desiring more data from their limited samples. In this application note, we show how you can use RePlex to detect and quantify pan and phospho isoforms of proteins such as those in the PI3K/Akt signaling pathway.
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A Multi-Antigen Serology Assay For COVID-19 Using Simple Western
2/4/2021
The characterization of the human immune response to SARSCoV-2 is central to understanding COVID-19 disease progression and the efficacy of a vaccine. In this application note, we show how the SARS-CoV-2 Multi-Antigen Serology Module for Jess/Wes from ProteinSimple allows for simultaneous detection of human serum IgG reactivity with 5 viral antigens commonly associated with COVID-19. This kit provides a richer view into the immune response to SARS-CoV-2 in a single, quick, and easy assay.
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Learn How Fully Automated Westerns Enable Cutting-Edge Targeted Protein Degradation Research
2/2/2021
To characterize the efficacy of Degrader molecules, researchers generally run dose response curves by way of traditional SDS-PAGE Western blotting methods. But the lengthy, manual workflow and resulting low reproducibility make it an unreliable approach for the determination of DC50 values. Instead, the ideal solution would be highly reproducible, allow for easy quantitation and have a short time to results. Simple Westerns are just that—they let you separate and analyze proteins by size (or charge) from 2 kDa to 440 kDa in just 3 hours.