A Multi-Antigen Serology Assay For COVID-19 Using Simple Western

The new SARS-CoV-2 Multi-Antigen Serology Module for Jess/Wes, which is run on capillary-based automated Western blot platforms from ProteinSimple known as Simple Western, can detect reactivity of human IgGs in serum or plasma with five key SARS-CoV-2 viral antigens simultaneously in a single capillary while also providing molecular weight information of each antigen, all without compromising sensitivity. The hands-free automation of Jess™ and Wes™ allows for ease-of-use and high repeatability, while minimizing time in the lab, which is ideal for social distancing measures. The runtime is quick, with only 3 hours to results, which may be analyzed remotely without returning to the lab. Plus, Simple Western platforms are flexible, open platforms, allowing users to incorporate their own proprietary antigens or even design entirely different immunoassays.

In this application note, we show how the SARS-CoV-2 Multi-Antigen Serology Module for Jess/Wes from ProteinSimple allows for simultaneous detection of human serum IgG reactivity with 5 viral antigens commonly associated with COVID-19, including the nucleocapsid, the spike protein, as well the spike protein’s S2, S1, and S1 RBD subunits. As reactivity with different antigens may indicate different stages of infectivity and neutralization, this kit provides a richer view into the immune response to SARS-CoV-2 in a single, quick, and easy assay.