INSIGHTS ON CELL & GENE THERAPY DISCOVERY

• Tumor-Infiltrating Lymphocytes: What, Why, And How

  As we’ve begun to understand the complexities of immune mechanisms through technology, we’ve found better ways to fight disease. Here, we focus on an important player in such targeted, highly personalized cancer therapies called tumor-infiltrating lymphocytes (TILs).

• Organs On Chips: Solving Persistent Pharmacokinetic Problems

  For many years, a rather insistent, expensive problem has been plaguing pharmacology — it’s quite tricky to understand the pharmacokinetics of new drugs without giving them to someone. Organs-on-chips, the specialty of Dr. Dan Tagle at the NIH, could be the answer.

• Using Preamplification To Maximize qPCR Data From Limited Samples

  Preamplification is frequently treated as a necessary evil as many fear that it may introduce bias into their experiments, leading to the generation of inaccurate results. Preamplification is also sometimes misunderstood as being a magic bullet to boost qPCR sensitivity. This article addresses these issues as well as others, so that you can be confident when using preamplification in your qPCR experiments.

• Robust Field Guide To qPCR

  qPCR remains the gold standard for validation of microarray and next generation sequencing data and the method of choice for both clinical and basic research labs for a wide range of applications. However, there remains general concern about the production of data that truly reflects the tested experimental conditions. We have developed a comprehensive guide to performing the ultimate qPCR experiment. The following is a snapshot of the critical steps needed to achieve excellent results.

• The Universal qPCR Reagent

  When quantifying nucleic acids via real-time or quantitative PCR (qPCR), high accuracy and reproducibility are essential. However, factors such as inconsistent pipetting and instrument design (specifically a lack of thermal or optical uniformity across the reaction plate) can lead to variations in fluorescence measurement.

• Codetection Of Viral Pathogen RNA And DNA Using One-Step Multiplex RT-qPCR

  RT-qPCR is a well-established method for the detection, quantification, and typing of different microbial agents in the areas of clinical and veterinary diagnostics, as well as food safety. There are multiple benefits of this technology. In this application note, we demonstrate a sensitive codetection of viral RNA and DNA targets in a multiplex setting using a novel one-step multiplex RT-qPCR supermix.

• Five Steps To Great Results Using One-Step Mulitplex RT-qPCR

  One-step multiplex RT-qPCR is a technique used to rapidly quantify multiple targets directly from RNA in a single reaction. This blog explains the proper optimization and validation for successful one-step multiplex RT-qPCR as well as tips for choosing the right reagents for your needs.

• Protein Thermal Shift Assays Made Easy

  Protein thermal shift assays enable quick and easy buffer optimization for increased protein stability. Learn more about the CFX Real-Time PCR Detection Systems which uses a simple protocol to measure protein thermal stability using SYPRO Orange Fluorescent Dye.

• Gene Selection Using PrimePCR Plates And CFX Maestro Software

  Reference gene selection and validation are very important for gene expression studies that involve different samples or experimental conditions and therefore are absolutely necessary before any further exploration. Read how PrimePCR Reference Gene Selection Panels provide an easy-to-use system with which to select appropriate reference genes.

• Trends In Protein Separation And Analysis — The Advance Of Stain-Free Technology

  Stain-free technology, a technology that existed in principle since early 2000 but was only commercialized in 2010 has changed the protein separation and analysis landscape in the past few years. This article focuses on how protein visualization is accomplished using stain-free technology, its advantages and concerns as well as how stain-free technology can change the way we carry out protein separation and analysis.