INSIGHTS ON CELL & GENE THERAPY DISCOVERY

• Using Avi-Tag Biotinylated Proteins To Determine Protein Interaction Kinetics And Affinity With Surface Plasmon Resonance

  Surface plasmon resonance (SPR) is a powerful, accurate, and direct way to measure protein interactions in real-time without the need for antibodies or detection reagents. In this study, we employ R&D Systems Avi-tag Biotinylated Proteins along with streptavidin Series S chips to specifically and uniformly capture proteins in a Biacore experiment, as an alternative to chemical or amine coupling.

• Evaluating Current Manufacturing Platforms For Recombinant AAV Production

  Realizing the full potential of viral vector-based therapies requires an understanding of the platforms currently available for recombinant AAV production as well as how to properly evaluate them.

• Preclinical CAR-T Cell Target Safety, Biodistribution, And Tumor Infiltration Analysis Using The RNAscope® ISH Assay

  In this report we illustrate a way to detect activated CAR-T cells in the tissue context, as well as low expression of CAR target antigen, using the RNAscope® in situ hybridization (ISH) technology.

• Liposome Testing

  Liposomes are spherical engineered particles made of phospholipids used in the pharmaceutical and cosmetic industries. The size and surface charge of liposomes are important characteristics which require measurement and monitoring. Dynamic light scattering (DLS) is the most common analytical technique used for measuring the size of submicron liposomes. Single particle optical sizing (SPOS) is used to measure the size of liposomes greater than one micron. The Entegris Nicomp® DLS system and AccuSizer® SPOS system are used in laboratories around the world for the accurate measurement of the size and charge (zeta potential) of liposomes.

• Nanoparticles Drive The Nanotechnology Revolution

  Nanotechnology has been referred to as potentially being the next technical revolution that could change the modern world. Perhaps the developments seen to date do not yet match this build up, but the combined investment by governments, universities, and industry is significant with global government investment exceeding $9 billion annually and growing. The United States government alone has invested over $27 billion since from 2001 – 2019.2 The global nanotechnology market has been estimated to be between $10 – 50 billion annually depending on definitions and sources.

• Visualization Of In Vivo Gene Editing Heterogeneity At The Transcript Level With The BaseScope™ Duplex RNA In Situ Hybridization Assay

  Genome editing, revolutionized by the CRISPR/Cas9 system, is a powerful research tool and promising therapeutic strategy for human genetic diseases. The BaseScope™ RNA in situ hybridization (ISH) Assay is the only method available to quantify cell-specific CRISPR-edited transcripts, including single nucleotide changes, in the context of intact fixed tissue. In this report we illustrate one use of the BaseScope™ Duplex Assay to assess the heterogeneity of in vivo CRISPR/Cas9 gene editing in the mouse liver.

• The End Of End Point Assays: How SPR Is Changing Analytics In Vaccine Design And Development

  Discover how SPR can be used to accelerate vaccine discovery and development by helping overcome analytical challenges that have plagued the industry for decades.

• Uniquely Powerful T Cells And How To Find Them

  T cell-based immunotherapy is promising as a treatment for multiple types of cancer but current techniques limit our ability to measure T cell characteristics of interest and select the most efficacious cells for a therapy. New technologies are needed to allow us to identify the drivers of desired phenotypes such as polyfunctionality, serial killing, and proliferation.

• Unravel The Complexity Of Single Cell Natural Killer Cell Cytotoxicity And ADCC Mediation

  Current experimental methods to characterize NK cells rely on bulk measurements of heterogenous samples that often contain contaminating cell types. In this application note, we demonstrate how NK cell function can be assessed at the single-cell level to characterize both cytotoxicity and ADCC mediation in the presence of target cells.

• Directly Link T Cell Phenotype And Function To Genotype

  Current techniques used to assess T cell function only allow us to draw correlative conclusions. This application note demonstrates how the Berkeley Lights platform can directly link phenotype to genotype to identify differences in CAR T cell killing kinetics as well as to recover TCR sequences that are associated with desired killing behavior.