INSIGHTS ON CELL & GENE THERAPY DISCOVERY
The Path To High-Quality Immunoassay Reagents
There is a continued demand for novel, fit-for-purpose immunoassays. Despite the challenges, it’s important for assay developers to streamline the selection and potential modification of critical reagents to get their tests to market quicker.
Utilizing The Multiparameter Capability Of The ZE5 Cell Analyzer To Monitor T Cell Exhaustion And Effects Of Immunotherapy
When working with limited patient samples, the ability to test multiple parameters simultaneously is highly valuable. This application note reviews how to successfully monitor in-depth changes to the T cell phenotype, exhaustion, and cellular subset identity markers using a 16-parameter panel developed for use with a 5-laser ZE5 Cell Analyzer. Using an in vitro model, they examined T cells before and after chronic stimulation, and in combination with a PD-1 receptor inhibitor, nivolumab.
Fast And Efficient Workflow To Monitor CRISPR-Cas9 Gene Editing Of Haploid Cell Lines
CRISPR's speed, low cost, and ability to be used in many model systems redefine what is possible in drug discovery and development. However, current workflows for generating knockout cell lines using CRISPR-Cas9 can be complex and inefficient. In this study, we demonstrate a novel workflow that includes Droplet Digital™ PCR, high resolution melt analysis, western blotting, and real-time PCR to generate and validate a knockout haploid cell line in less than five weeks.
Ultra-Sensitive Quantification Of Genome Editing Events Using Droplet Digital™ PCR
Genome editing has become a widely used tool and allows specific modification of almost any cell’s genome. However, the often low (<5%) frequency of gene edits, in particular for some primary or induced pluripotent stem cell types, hinders facile and reliable detection by gel- or sequencing-based methods, or requires laborious clonal isolation of edited cells. Here we introduce an ultra-sensitive, cost-efficient Droplet Digital PCR–based method for the detection of HDR and NHEJ alleles generated using the CRISPR/Cas9 and TALEN genome editing systems.
Utilizing The ZE5 Cell Analyzer To Monitor T Cell Exhaustion And Effects Of Immunotherapy
Efficient monitoring of T cell surface markers, cytokines, and expression patterns is critical to the study of immunotherapies. Learn how the Ze5 Cell Analyzer enables you to test these multiple parameters simultaneously.
Scale-Up Of CHO Cell Fed-Batch Cultures In HyClone™ ActiPro™ Medium Supplemented With Cell Boost™ 7a And 7b
The most widely used cell lines for biopharmaceutical production of therapeutic proteins originate from CHO cells. These cells are robust in culture and are able to produce a variety of recombinant glycoproteins at high levels in large scales. This application note compares cell growth and recombinant protein production of multiple Chinese hamster ovary (CHO) cell clones when cultured in HyClone ActiPro basal medium and Cell Boost 7a and 7b feeding supplements.
Effects Of Feeding Strategy On CHO Cell Performance In Fed-Batch Cultures Using HyClone™ ActiPro™ Medium And Cell Boost™ 7a And 7b Supplements
ActiPro cell culture production medium is intended to be used in combination with Cell Boost 7a and 7b supplements to enhance recombinant protein production in fed-batch or perfusion processes. This work demonstrates the development of a fed-batch process for a CHO cell line producing a monoclonal antibody (mAb). The results emphasize the importance of an appropriate feeding strategy to achieve optimal cell growth and productivity.
Optimizing Cell Culture Media To Increase Cell Culture Titer With Maintained Protein Quality
When evaluating media with the goal of increasing titers, it is important to ensure that protein quality is not negatively impacted. So how can this process improvement be performed reliably? It makes sense to use a Design of Experiments (DoE) and empirical approach. Here is an example of how to do it.
Enhanced Host Cell Protein Analysis In Biologics Manufacturing
The most widely used and accepted method to monitor host cell protein levels in biologics is an immunological assay, Enzyme-Linked Immunosorbent Assay, also known as ELISA. However, this coverage assay comes with some known challenges. Learn more about an enhanced anti-host cell protein antibody coverage analysis method solution.
Purifying Tagged Proteins Using ÄKTA Go Protein Purification System
ÄKTA go is a small and compact liquid chromatography system that allows researchers to perform routine protein purification with ease while allowing for efficient use of bench and cold cabinet space.