Frost & Sullivan recently invited academic and industry leaders in neuroscience research to participate in a unique thought leadership forum, our Virtual Think Tank series, titled Biomarkers for the Brain. This forum brought together leading minds involved in the study of biomarkers of central nervous system (CNS) diseases and disorders. The Virtual Think Tank stirred discussion about technological advancements in the field, current progress, future implications of the research and development occurring today, plus unmet needs in tools, key challenges that still remain, and expectations for future usage of biomarkers in CNS diseases.
Industry experts joined a panel at Frost & Sullivan's Virtual Think Tank recently to discuss the current state of immunotherapies, implications of research and development occurring today, key challenges, and future approaches to the targeted use of the immune system in cancer treatment. Read their thoughts on immunotherapies and the critical role for the immune system to play, especially in the advanced stages of cancer, since traditional standard-of-care therapies do often work fairly well.
Read these tips to help maximize cell separation and isolation and set you up for downstream processes, including cell sorting.
Helpful tips such as carefully choosing the most suitable fixative and permeabilization reagents as well as other factors that should be considered when designing intracellular flow experiments.
The most common method of exosome analysis by flow cytometry is using magnetic beads for capture and detection. This poster presents both methods of staining and instrument setup, and details for direct exosome detection on a ZE5 using single and dual triggers.
Planning your flow cytometry experiment can be time consuming. With this in mind, this new poster aims to help you plan your experiment by going through the key steps you need to consider before you start.
Reference this poster as a fluorophore reference guide to help you plan your flow cytometry experiment. You can find essential information for common flow cytometry fluorophores including optimal laser excitation, maximal emission and relative brightness, as well as handy tips for panel design.
In this study, a S3 Cell Sorter was used to analyze and purify prospective MSCs from passage 1 (P1) and passage 3 (P3) bone marrow cultures. The data presented here shows the capability of the S3 Cell Sorter to isolate rare MSCs from a mixed bone marrow culture with satisfying sort efficiency and postsort purity.
A demonstration of a simple workflow to verify the reliability of the S3e Cell Sorter in sorting single
cells. Using this method, a confirmed sorting accuracies of >94% and 100% for single cells and single beads is achieved, respectively.
Compare flow cytometry–based exosome detection methods using the ZE5 and demonstrate that exosomes can be directly detected without being attached to beads.