• Sort Cells Using Data In Real Time

    Researchers working in flow cytometry require the highest-quality data from every sample in order to achieve increasingly challenging scientific goals. The Bigfoot Spectral Cell Sorter’s acquisition and sorting application, Sasquatch Software (SQS), allows the operator to sort and analyze either spectrally unmixed data or traditional compensated data. In this paper, we will demonstrate a cutting-edge technique of combining spectral workflow and multiparameter six-way cell sorting.

  • Multi-Sample Loader Offers Improved Sorting And Analysis Of Cells

    As studying 20 or more fluorescence parameters is becoming increasingly common in flow cytometry, a spectral cell sorter with a multi-sample loader can offer better functionality. This paper explains how the Bigfoot Spectral Cell Sorter can acquire samples on average within 9.89 seconds, excluding the time for rotation to the sample station, which varies with the location of each position.

  • Options For Light Scatter And New Opportunities For Research

    The field of flow cytometry is rapidly maturing, and instrumentation is constantly improving. However, many researchers are frustrated by instrument limitations that make it difficult or impossible to observe small particles that are likely present in samples, yet are undetectable. This paper characterizes the use of unique light scatter parameters and describes additional features such as below-the-trigger-threshold detection, extremely low background signal, and superior electronics that make the Bigfoot Spectral Cell Sorter well suited for micro- and nanoparticle research.

  • High-Throughput Plate Sorting

    Plate sorting is accomplished through movement of a stage that holds the plate, positioning it directly under a sort stream that will deliver a cell into each well. Because of the requirement for precise targeting, the plate deposition mechanism requires careful calibration and alignment. In this paper a variety of tests using the horseradish peroxidase (HRP) method were performed on the Bigfoot Spectral Cell Sorter to test deposition accuracy and precision, robustness, and speed of the hardware.

  • A New Approach To Cell Sorter Safety

    Cell sorting continues to be a powerful contributor in the quest to improve human health, allowing researchers to make timely advancements in drug and vaccine development. However when cell sorters are run at high pressures, they can produce aerosols with high particulate content as well as extremely small particles. While several newer sorters include systems to address this concern they continue to resemble an ad hoc solution with manual operation of air-handling controls and poor access for service manipulation, which can lead to inconsistent results and an unsafe environment. In this paper we review a cell-sorter with a design to solve these problems.

  • High-Throughput Automation With The Attune NxT Autosampler

    The emerging field of high-throughput (HT) flow cytometry is extending the capabilities of cell-based screening technologies into the profiling of compound libraries. High-throughput flow cytometry is a particularly useful approach for screening compounds in cell models where multiple readouts are desired. In this application note we examine the Attune NxT Flow Cytometer with Attune™ NxT Autosampler, which uses a unique volumetric sample and sheath fluid delivery system.

  • Nanogold Particles Using The Nicomp® System

    Gold nanoparticles, also sometimes called colloidal gold, possess unique properties that are beneficial to many applications. The size of gold nanoparticles is a critical physical parameter requiring careful measurement. The most popular technique for particle size and zeta potential analysis is dynamic light scattering (DLS) and electrophoretic light scattering (ELS). This application note demonstrates how the Nicomp® Z3000 system is ideally suited for determining size and zeta potential of gold nanoparticles.

  • Streamlining And Standardizing Cell And Gene Therapies From Process To Product

    Cell and gene therapies are transforming the lives of patients with various rare genetic diseases and more common conditions such as cancer. However, the rapid pace of scientific progress has overwhelmed supply infrastructure, creating pressures to scale manufacturing, increase capacity and meet a wide range of deliverable requirements.

  • Corning® X-LAB® System For Closed, Sterile PBMC Recovery

    Research and therapies involving the human immune system rely heavily on peripheral blood mononuclear cells (PBMCs) isolated from whole blood sources. The Corning X-LAB system is designed to isolate and harvest PBMCs from blood and blood products in a closed, sterile device that is automation-friendly. Here we show how the Corning X-LAB system can deplete more than 99% of red blood cells and 80% of platelets.

  • Fundamentals Of Size Exclusion Chromatography

    Size exclusion chromatography (SEC), also known as gel filtration, is the mildest of all chromatography techniques. SEC separates molecules by their differences in size as they pass through a resin packed in a column. You can use SEC for both preparative and analytical studies of your protein. But how do you know which parameters will give you the best resolution of your protein mixture?