Bio-Techne Application Notes

41. See What's Inside Your AAV Capsids 11/23/2021

    How to quickly develop a method to characterize and assess the stability of empty, intermediate, and full AAVs, providing a critical, quality solution for safe, fast, and effective gene therapy development.

42. Fast And Reproducible AAV2 Quantification With Automated ELISA 7/30/2021

    As part of the viral quantification process, researchers rely on various analytical methods to assess critical quality attributes. While traditional plate-based and dot blot assays can be useful, they are often complex, time-consuming, and non-standardized. In this application note we demonstrate how AAV2 capsid titer quantification with the Simple Plex AAV2 assay is a robust alternative to the conventional ELISA or dot blot approaches.

43. Assess The Purity Of Your Cell Therapy Product With Confidence 6/4/2021

    The analysis of particle contaminants in cell therapy products is critical for ensuring drug purity and safety. However, the successful characterization of such particulates requires gaining morphological insights into each type of particle present. A technique called micro-flow imaging (MFI) enables the visualization of particulate matter by providing relevant morphological details for further study of each type of particle identified. In this study, MFI was used to distinguish between NK cells and its expansion component, Cloudz™ microspheres, in different environments.

44. Advance Your Western Workflow Into The 21st Century With Abby 5/18/2021

    Protein analysis comes with many challenges, typically requiring labor-intensive, time-consuming protocols like the Western blot, which has multiple hands-on steps that increase user error and data variability. Abby™ is the latest Simple Western™ instrument dedicated to changing the way scientists analyze proteins by eliminating common protein analysis workflow challenges. Here we show how Abby delivers picogram-level sensitivity, while the RePlex assay enables detection of multiple targets per sample as well as total protein detection.

45. Concentrating On AAV Impurities With Ultrasensitive Total Protein Detection On Simple Western 4/6/2021

    Impurities in protein products can be dangerous and impact efficacy. For example, protein impurities in final drug products could lead to undesirable immune responses in patients, so detecting total protein is critical for revealing impurities in preparative protein production. Simple Western™ assays on instruments like Jess and Wes from ProteinSimple offer fully automated protein separation and quantification with small sample volumes, and sensitive chemiluminescent-based immunodetection and total protein detection.

46. Tissue Biodistribution Of AAV-Based Gene Therapy With The RNAscope® Assay 3/3/2021

    AAV tissue biodistribution and cell tropism are essential pieces of information for every gene therapy development program. The RNAscope® ISH assay is a highly informative method for quantitative, cell-specific measurement of AAV vector and transgene expression.

47. Using Avi-Tag Biotinylated Proteins To Determine Protein Interaction Kinetics And Affinity With Surface Plasmon Resonance 3/2/2021

    Surface plasmon resonance (SPR) is a powerful, accurate, and direct way to measure protein interactions in real-time without the need for antibodies or detection reagents. In this study, we employ R&D Systems Avi-tag Biotinylated Proteins along with streptavidin Series S chips to specifically and uniformly capture proteins in a Biacore experiment, as an alternative to chemical or amine coupling.

48. Monitoring Target Engagement In Drug Discovery: Application Of Wes To The Cellular Thermal Shift Assay 3/2/2021

    In drug discovery, confirmation of in-cell target engagement is a critical component of the drug development process. In this application note, quantitative and reproducible  CETSA data generated with ProteinSimple’s Wes™ instrument (CETSA-Wes) are presented. This assay verifies drug–target binding, and given its quantitative nature, the half maximal inhibitory concentration (IC50) is also calculated.

49. Preclinical CAR-T Cell Target Safety, Biodistribution, And Tumor Infiltration Analysis Using The RNAscope® ISH Assay 3/1/2021

    In this report we illustrate a way to detect activated CAR-T cells in the tissue context, as well as low expression of CAR target antigen, using the RNAscope® in situ hybridization (ISH) technology.

50. Visualization Of In Vivo Gene Editing Heterogeneity At The Transcript Level With The BaseScope™ Duplex RNA In Situ Hybridization Assay 2/23/2021

    Genome editing, revolutionized by the CRISPR/Cas9 system, is a powerful research tool and promising therapeutic strategy for human genetic diseases. The BaseScope™ RNA in situ hybridization (ISH) Assay is the only method available to quantify cell-specific CRISPR-edited transcripts, including single nucleotide changes, in the context of intact fixed tissue. In this report we illustrate one use of the BaseScope™ Duplex Assay to assess the heterogeneity of in vivo CRISPR/Cas9 gene editing in the mouse liver.