Development beyond traditional monoclonal antibodies (mAbs) is increasing, and today, we see antibody variants like multi-specific and bispecific antibodies and antibody fragments.
In mAb processing, affinity chromatography is used to capture the target molecule based on specific interactions between the chromatography resin and the target molecule. For traditional mAbs, protein A chromatography is used to capture antibodies. Depending on the structure of the target antibody and the impurity profile, other affinities, like protein L or variants of protein A, may improve purity and increase removal of impurities. It is important to select a capture resin based on the target molecule.