Diversification of antibody molecules requires a toolbox of resins
Development beyond traditional monoclonal antibodies (mAbs) is increasing. Today, we see antibody variants such as multispecific and bispecific antibodies and antibody fragments.
In mAb processing, affinity chromatography is used to capture target molecules based on specific interactions between the chromatography resin and the molecule. For traditional mAbs, protein A chromatography is used to capture antibodies. Depending on the structure of the target antibody and the impurity profile, other affinities such as protein L or variants of protein A may improve purity and increase the removal of impurities.
This guide helps you select a capture resin based on the target molecule.