MabSelect PrismA™ Protein A Chromatography Resin

Source: Cytiva
MabSelect PrismA™ Feature

Protein A chromatography resin with enhanced alkaline stability and binding capacity for improved process economy in antibody purification.

MabSelect PrismA™ resin is available for immediate delivery.

MabSelect PrismA™ affinity chromatography resin has been improved with an optimized high-flow agarose base matrix and a genetically engineered protein A-derived ligand. This allows future demands in mAb purification to be met, including processing of many bispecific antibodies.

  • Enhanced dynamic binding capacity allows high mass throughput of processed mAb per resin volume unit.
  • Excellent alkaline stability enables efficient cleaning and sanitization using 0.5–1.0 M NaOH for improved process economy, bioburden control, and robustness.
  • Covered by a comprehensive security of supply program, including dual sources of the agarose base matrix and protein A ligand.
  • The PrismA protein A ligand is also available in HiTrap Fibro™ and HiScreen Fibro™ PrismA chromatography units for ultrafast purification of mAbs.
  • Easily measure protein A leakage using the PrismA ELISA kit specifically designed for use with MabSelect PrismA™ resins.

The enhanced alkaline stability of MabSelect PrismA™ also provides opportunities for more robust mAb purification using periodic counter-current chromatography. The possibility for cleaning using 1 M NaOH increases bioburden control for continuous processes, which can be exposed to days or weeks of feed harvest.