Application Notes

  1. Clarifying And Sterile Filtering Cells For Protein Purification Workflows 12/11/2020

    In recent years, biopharmaceutical research processes have demonstrated major improvements in the quality and recovery of mAbs, which to an extent have been associated with culturing the expressing cell lines at high cell densities. This work has generated a great challenge in cell clarification, sterilization, and further downstream processing. These processes must remove large amounts of biomass and increased levels of contaminating cell debris generated during cell culture and harvesting.

  2. Alternatives For Low- And High-Throughput RNA Isolation/Extraction 12/11/2020

    This paper demonstrates that extraction column spin devices can efficiently isolate and purify high-quality RNA and genomic DNA with yields and qualities similar to devices found in commercial kits.

  3. The Demand For High Quality DNA Necessitates Improved Techniques for High-Throughput Purification 12/11/2020

    The demand for high purity plasmid DNA continues to increase in molecular biology research. This app note covers a solution for nucleic acid binding for small scale plasmid DNA purification.

  4. 24-Well Filter Plates Offer Alternative For Clarifying Mammalian Cell Cultures 12/11/2020

    Centrifugation and filtration have been widely accepted as techniques required for clarifying complex cell cultures to recover extracellular proteins such as monoclonal antibodies (mABs). Read an alternative to the use of centrifugation/filtration/flocculation to clarify mammalian cells cultures and describes assessment of newly developed AcroPrep 24-well clarification and sterilization plate and AcroPrep 24-well sterilization plate for clarification of mammalian cell cultures (2 to 26 x 106 cells/mL) and recovery of proteins present in the supernatant.