Application Notes

  1. DNA Purification From PCR Or Agarose Gels 4/28/2022

    This study assesses the use of NAB Nanosep centrifugal devices with commercially available reagents for PCR or agarose gel cleanup.

  2. How To Choose The Correct Syringe Filter For Analytical Sample Preparation 4/28/2022

    This paper simplifies syringe filter selection and reviews the membranes and sizes required for specific analytical applications.

  3. New Filtration Protocols Accelerate Viral Vector Development And Enhance Scale Up To Production 3/15/2022

    Read how filtration technology is optimizing a scalable and GMP-compatible purification process for lentiviral vectors that works effectively from research and development through manufacturing.

  4. SARS-CoV-2 Viral RNA Extraction From Human Saliva Samples With NAB Nanosep® Centrifugal Devices 9/13/2021

    In this study we evaluate the performance of the Pall NAB Nanosep centrifugal device for the isolation of SARS-CoV-2 viral RNA from human saliva samples spiked with heat inactivated virus using reagents (RCOM1 and RCOM2) from two commercially available kits for extraction of viral RNA.

  5. Lysate Clearance To Remove Cellular Debris 9/13/2021

    The most problematic step in the purification of synthesized biomolecules is the clarification of the sample once the cells are lysed. The lysate often contains biomolecule concentrations millions of times higher than the molecule of interest. Here we present a lysate sample preparation procedure using the AcroPrep™ Advance filter plate for lysate clearance that effectively removes unwanted cellular debris from samples.

  6. Combining Cell Clearance And Sterile Filtration Functionality For Improved Clarification Of Mammalian Cell Cultures 9/13/2021

    This application note demonstrates systems that have proven quite useful for clarification of mammalian cell cultures such as CHO-cells, by combining the cell clearance and sterile filtration functionality in a single device.

  7. Achieve Ultrafiltration, Microfiltration And Macrofiltration With 24-Well Filter Plates 2/17/2021

    Many techniques in Life Science research rely on efficient sample preparation methods. Concentration and purification of samples are key steps in the workflow that can greatly affect the outcome of the experimental results. Pall has increased the range of AcroPrep 24-well filter plates offered to help streamline membrane selection and provide effective solutions for multiple sample preparation needs. This document reports the data generated by ultrafiltration of proteins of known size with AcroPrep 24-well filter plates.

  8. From Cell Culture To Target Protein: Exploring The Benefits Of The 24-Well Filter Plate Workflow 2/12/2021

    Over the last 5 years, the use of 24-well cell culture plates in laboratory applications has substantially increased. This market segment has expanded at an estimated 14% compound annual growth rate (CAGR)— a pace that is expected to continue in coming years. This paper explores the evaluation of the potential applications for its new AcroPrep 24-well filter plate line, plus the time savings it can achieve in protein purification workflows.

  9. Filtration Of Viscous Materials For Microbial QC 2/12/2021

    Membrane filtration is the preferred method for sterility testing. Viscous products, such as oils, creams and ointments can prove difficult to filter. As per USP <71> viscous oils may be diluted as necessary with a suitable sterile diluent such as isopropyl myristate shown not to have antimicrobial activity in the conditions of the test. Sterile isopropyl myristate (IPM), has been shown to have no anti-microbial activity in test conditions. This study investigates the effectiveness of IPM as a diluent by comparing filtration times with unfiltered oils.

  10. Alternatives For Low- And High-Throughput RNA Isolation/Extraction 12/11/2020

    This paper demonstrates that extraction column spin devices can efficiently isolate and purify high-quality RNA and genomic DNA with yields and qualities similar to devices found in commercial kits.