OptiPEAK T Lymphocyte XPR Expansion Of T Cells Without Serum In Chemically Defined Media

By Atherly Pennybaker, Media Formulation and Product Applications Specialist, InVitria

Major components used to expand human T cells include human and bovine serum, as well as proteins derived from these substances. These components not only have the potential to introduce harmful pathogens to the already compromised patient in a clinical setting, but they also have significant limitations in the ability to ensure robust and reproducible performance in cell culture systems.

OptiPEAK T Lymphocyte XPR media (product No. 555CDK0045-1L) supports the growth and expansion of human primary T cells and contains only synthetic and recombinant components. This medium is considered blood-free and all components are chemically defined, providing robust performance without compromising growth, safety, or phenotype.

Here we demonstrate how OptiPeak T Lymphocyte blood-free medium’s potential as a viable and regulatory-friendly option for T cell immunotherapy manufacturing.