What Is Size Exclusion Chromatography (SEC)?

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Monoclonal antibodies have transformed biotherapeutic development, but their size and structural complexity make quality control especially demanding. One of the most important attributes to monitor is aggregation, which can occur at multiple points during manufacturing, storage, or formulation. Even low levels of aggregates may affect safety, efficacy, and regulatory acceptance, making reliable analysis essential before these therapies reach patients. Size exclusion chromatography (SEC) remains a trusted method for aggregate analysis because it separates molecules based on size while preserving their native state.

Learn how SEC supports antibody characterization, helps identify aggregation risks, and provides the data needed to strengthen confidence in biotherapeutic drug development.