TrypLE Select Enzyme: A Temperature-Stable Replacement For Animal Trypsin In Cell Dissociation Applications

The challenges of working with animal-derived trypsin, including its inherent instability, can complicate routine cell culture. For researchers seeking a reliable and stable alternative, TrypLE™ Select Enzyme offers a significant advancement in cell dissociation. This high-purity, recombinant enzyme demonstrates remarkable stability, remaining active for over 12 months when stored at room temperature or 4∘C. Unlike porcine trypsin, which loses more than 95% of its activity within 24 hours at 37∘C, TrypLE™ Select maintains 85% activity for 8 days at the same temperature.

Furthermore, HPLC analysis confirms its purity with a single peak, a stark contrast to the multiple peaks indicating impurities in animal trypsin preparations. This superior stability and purity streamline handling and storage for routine cell culture.

To see the full stability data and purity analysis, read the complete application note.