Testing Liquid Handler Performance With PCRMix

Master mix is a critical component in any PCR-based assay such as qPCR and NGS library prep. However, it behaves very differently compared to regular aqueous solutions, and different liquid class settings are required for accurate volume delivery.

The amount of master mix added to your reaction can directly affect a qPCR assay’s Cq value which correlates to the number of cycles needed to detect a real signal from a sample. In this video, we demonstrate the importance of using an appropriate test solution when optimizing a liquid handler to dispense the master mix.