Successful Development Of A Scalable And Robust Process For MSC-EV Production

By Stephen Lenzini, Jae Jung, Grishma Patel, Sanket Jadhav, Priyanka Gupta, Rukmini Ladi, Michael Boychyn, Jon Rowley, and Elie Zakhem

Mesenchymal stromal/stem cell-derived extracellular vesicles (MSC-EVs) are generating increasing excitement in regenerative medicine and therapeutic development. However, scaling EV production from lab to clinic remains a major hurdle. RoosterBio has tackled this challenge by engineering a robust, scalable upstream process using xeno-free MSCs, optimized media, and stirred-tank bioreactors. The resulting process delivers consistent, high-yield EV harvests at both small and large scales, with confirmed particle identity, biofunctionality, and potency.

Download the poster to discover how this process was developed, validated, and scaled to 15L – and where it’s heading next.