Streamlined Single-Injection Middle-Down Workflow Using EAD For Biotherapeutics Characterization

By Haichuan Liu and Zoe Zhang SCIEX, USA

A streamlined middledown workflow using electron-activated dissociation (EAD) allows you to achieve high sequence coverages (70%-80%) of monoclonal antibody (mAb) subunits in a single injection. This type of workflow combines the descriptive and reproducible fragmentation provided by an EAD mechanism with automated data analysis using Biologics Explorer software.

Key features of the EAD-based middle-down workflow include:

• High sequence coverage
• A streamlined workflow for simple sample preparation, efficient data acquisition and automatic data analysis
• A single-injection method for routine analysis of mAbs
• Reproducible fragmentation and detection of low-abundant fragments

Review data and studies that show how the ZenoTOF 7600 system and Biologics Explorer software provides an easy-to-use and optimized workflow template for improved user experience with middle-down data analysis.