Single Extracellular Vesicle Profiling: Harnessing NanoFlow Cytometry For Quantitative Surface Marker Analysis

Traditional bulk analysis methods for extracellular vesicles mask critical heterogeneity across individual particles, leaving surface marker expression patterns poorly understood. Single-vesicle analysis through calibrated NanoFlow cytometry offers unprecedented resolution to quantify surface markers at the molecular level across EV populations.

This scientific poster presents a novel quantitative approach for characterizing CD81, CD63, and CD73 expression on highly purified mesenchymal stem cell-derived EVs from three tissue sources. Discover how single-particle profiling reveals tissue-dependent and marker-specific expression patterns that bulk methods cannot detect. The methodology converts arbitrary fluorescence values to precise molecule counts per particle, establishing lower limits of detection for each surface marker. Learn how expression frequency, molecular abundance, and size distributions differ dramatically between tetraspanins and tissue origins.

Download the poster below to explore the complete quantitative datasets, including percentile distributions, violin plots revealing expression heterogeneity, and insights into how CD73 preferentially associates with larger EVs while CD63 and CD81 dominate smaller particles.