Single-Cell Dispensing And Screening Of Cell Lines For Monoclonality Verification

By Prathyushakrishna Macha, Chen Wenyi, Rebecca Kreipke, Sheela Muley, Oksana Sirenko, Janet Graystone | Molecular Devices, LLC David Bonzon, Georges Muller | SEED Biosciences

Use the impedance-based single-cell dispenser and high-throughput fluorescence-based imager to optimize gene editing, single-cell dispensing, and screening during the monoclonal cell-line development process. Tests have proven that combining of DispenCell™, the single-cell dispenser, and the CloneSelect® Imager FL workflow provides an innovative and simple impedance-based method for the isolation of intact single cells and imaging. Review the results of methods used to improve single-cell dispensing workflows, CHO cell dispensing and imaging, and HEK-293 colony outgrowth and screening.