Review Of mRNA Vaccines & Therapeutics Manufacturing Challenges - mRNA Manufacturing

By Dr. Catherine Jomary, Bioprocess Cell & Gene Technologist

Over 30 years ago, mRNA was identified as a potential vaccination approach against cancer. In spite of its sensitivity to the almost ubiquitous ribonucleases and its rapid degradation in situ, mRNA offers strong safety advantages. The full mRNA manufacturing process presents several challenges to be overcome. In this review we focus on defies associated with mRNA attributes, (i) its high sensitivity to the virtually omnipresent ribonucleases, (ii) its high sensitivity to shearing, and (iii) size similarity with some impurities (double-stranded RNA, or dsRNA). Learn more about the synthesis, purification, and encapsulation of mRNA and the challenges each step brings.