Reliable Single Cell Outgrowth: Dark Art Or Science

Single cell cloning (SCC) is a critical and high value process for an increasing number of applications including stable cell lines for therapeutic mAbs and biosimilars; stable cell lines for viral vector production for gene therapy; and will become increasingly important for cell therapies.

Here, we discuss how the VIPS™ (Verified in-situ Plate Seeding) system, in combination with the InstiGRO™ single-cell cloning supplements (from SAL Scientific), offers customers a high-efficiency solution which ensures plates are populated with high numbers of healthy single cells that grow into resultant colonies for screening, expansion and ultimately selection for master cell banks.