Osmolality Analysis In Bioprocessing: From Theory To Practice

In biopharma, osmolality serves as a critical quality control (QC) check for cell culture media, buffers, and formulated drug products. Moreover, it is utilized as a critical process parameter during upstream cell culture and downstream filtration. This informative webinar explores the impact of osmolality in bioprocessing and its correlation with the production of immunoglobulin G (IgG). The featured speaker will describe the correlation between osmolality assessment and IgG production when examining the effect of plant protein hydrolysates as additives, in a media development project.

In the investigation into the recoverability of cells at varying stages of nutrient depletion, osmolality measurements were employed to evaluate the suitability of culture conditions in ‘re-fed’ media compared to control conditions with complete media. These preliminary investigations are in the process of being scaled up to the bioreactor level (bench scale) where osmolality validation will be applied to samples collected through at-line process analytical technology (PAT) instrumentation development.