Optimal Storage Of Murine Tumors In MACS® Tissue Storage Solution

By Janina Brauner, Laura Nadine Küster, and Olaf Hardt, Miltenyi Biotec

The storage of fresh organs and solid tissue samples is often required in research projects when shipping samples to and from external sources, when samples need to be collected at different times, or simply when samples cannot be processed all at once. In these cases, short-term storage allows full flexibility for further processing of the tissues within a few days. For the analysis of viable cells from the stored tissues, it is essential to avoid necrosis and apoptosis during the storage period. Additionally, cell activation must be prevented, as well as changes in pluripotency of cells to preserve the physiological and functional status of the cells. A common storage method is to freeze tissue samples, but this frequently leads to high cell death after thawing and is a challenge for shipping.

A tissue storage solution has been specifically developed to overcome these issues that allows the optimal storage of fresh solid tissues for at least 48 hours at 2–8 °C. Within this storage period, cell viability, functional and activation status of cells are preserved, and induction of cellular stress-related genes is prevented. See how this solution performed in the preservation of murine tumors.