One-Step Isolation And Activation With Flexible Active Release To Optimize T Cell Phenotype

Delivering a higher-quality pool of early T cell phenotypes is key to improving the reliability and longevity of therapeutic outcomes. To aid in the production of the desired phenotypes, the CTS Detachable Dynabeads CD3/CD28 Kit provides control of activation with user-defined dissociation of the active-release beads at any time after activation. This feature was designed to help preserve an early T cell phenotype and shorten the T cell manufacturing process to a few days.

In this study the CTS Detachable Dynabeads CD3/CD28 Kit was evaluated in a comparative bench-scale study to assess the performance of T cell activation as well as post-activation cell expansion, CD4+:CD8+ ratios, and early phenotype, relative to the passive release beads. Another study was conducted with the active release beads in the CTS DynaCellect Magnetic Separation System to evaluate T cell purity, isolation efficiency, and activation with bead release on different days post-activation.