Application Note

N-Linked Glycan Analysis Of NISTmAb And All-Inclusive Sample Preparation

By Marcia Santos and Fang Wang, SCIEX, USA


The Fast Glycan Labeling and Analysis kit for the BioPhase 8800 system demonstrates high-throughput N-linked glycan analysis of the NIST monoclonal antibody (NISTmAb), trastuzumab and etanercept. Utilize this accurate and reliable workflow for all of your sample preparation needs from denaturation, digestion, labeling and cleanup, to data analysis. Using the BioPhase software, this kit performed reliable glycan peak identifications with average GU %RSD values lower than 0.06% for all three proteins tested in this application study. Achieve a 1-minute-per-sample analysis rate, along with streamlined and high-throughput glycan analysis assay for all phases of biopharmaceutical development.

Key features of released N-linked glycan analysis

  • High-throughput glycan analysis workflow: The workflow can achieve a 1-minute-per-sample analysis rate.
  • Intuitive and flexible data analysis software: BioPhase software provides automatic peak identification for common N-glycans. It also allows customers to customize their glycan libraries based on molecule type.
  • All-inclusive sample preparation in one kit: The Fast Glycan Labeling and Analysis kit provides all the chemicals and enzymes needed for N-glycan analysis.
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