Monitoring And Determining The Cause Of Antibody Discoloration Using Capillary Isoelectric Focusing And Electron Activated Dissociation

By Haichuan Liu, Marcia Santos, Andy Mahan, Hirsh Nanda, Kristen Nields, Chelsea Leonce, and Zoe Zhang, SCIEX, USA; Janssen, USA

View a study which provides additional insight into the factors that govern the discoloration of protein therapeutics and the role of AGEs in color change.

Color is a potential critical quality attribute of protein therapeutics. Undesirable color changes can lead to increased product heterogeneity and concerns about the safety and efficacy of a drug. Here we will highlight a streamlined solution that combines the power of cIEF to monitor antibody discoloration and an EAD-based MAM workflow to determine the cause of color change.