Mo-DC Generation: From Monocyte Isolation To DC Characterization

Dendritic cells (DCs) are professional antigen-presenting cells that are essential for the adaptive immune system. They can take up tumor-associated antigens and present them to T cells, which leads to the activation of T cells and the production of antibodies. To obtain functional monocyte-derived DCs (Mo-DCs) that possess the distinct phenotype and function of natural DCs, it is crucial to follow reliable procedures using high-quality reagents.

The generation of Mo-DCs can be achieved by using various protocol formats, manually and automatically, at various scales. In this application note, we describe the procedure for 6-well plates as an example. Read through the steps involved in generating functional monocyte-derived DCs (Mo-DCs), and explore results that demonstrate the Miltenyi Biotec products that can be used to generate functional Mo-DCs, suitable for a variety of applications.