Molecular biology methods have led to the successful production of a variety of synthesized biomolecules (DNA, RNA, proteins) from prokaryotic and eukaryotic cell lines. The most problematic step in the purification of these biomolecules is the clarification of the sample once the cells are lysed. The lysate often contains biomolecule concentrations millions of times higher than the molecule of interest.
In the past, centrifugation was the primary method used to sediment cellular debris. Sedimentation has several limitations for many applications where small-scale, high-throughput processing is required.
Filtration, which is easily automated, is relatively quick and allows the use of additional wash steps to maximize sample recovery. Filtration can be done effectively in either vacuum or centrifugal mode, ultimately maximizing the choice in protocols available to the researcher.
We present a lysate sample preparation procedure using the AcroPrep™ Advance filter plate for lysate clearance that effectively removes unwanted cellular debris from samples.