Leveraging dPCR r Techniques To Quantitate Lentivirus Particles

In recent years, digital PCR (dPCR) technology has become a crucial tool for the absolute quantification of nucleic acids across various fields, from bioproduction to diagnostics. This technique has proven to reduce variability and increase the accuracy and analytical sensitivity of your data, even in high-background conditions.

For lentiviral samples, you can use dPCR to measure vector titer and provirus copy number in transduced cells. By leveraging LTR-based quantitation, you can even enable the correlation of total (genomic) and infectious viral particles, which is a key component of lentiviral characterization to comply with regulatory guidance. Watch this on-demand webinar to learn how you can use a combination of commercially-available kits, instruments, and software to obtain sensitive and accurate data without sacrificing time and resources.