By Anne Richter, Ramona Siemer, Elvira Criado-Moronati, Anna Baranska, Philipp Gert, David Agorku, Olaf Hardt, Daniela Vorholt, Aparajita Singh, Ruth Kläver, Christian Dose, Bianca Heemskerk, and César Evaristo, Miltenyi Biotec, Bergisch Gladbach, Germany
Explore a new workflow for the isolation and analysis of tumor-infiltrating lymphocytes (TILs). This new workflow includes tissue storage, tumor dissociation, dead cell removal, and cell isolation to enrich viable T and B cell populations. T and B cells were successfully isolated, significantly reducing the time required for flow cytometric analysis and flow sorting of rare cell populations, and single-cell sequencing of TILs was improved.
Here, we look further into the study that was conducted to show how this workflow could be a valuable tool for researchers studying immunotherapy.