Improving Expression And Purification Of mAb Using FectoCHO® Expression System And MabXpure™

Transient gene expression (TGE) is commonly used for medium scale production of recombinant proteins and antibodies in suspension CHO cell lines. This approach allows production of sufficient protein amounts for “proof of concept” studies or tools validation, prior to moving on to time-consuming, but cost-effective production of stable cell lines at large scale. Speed and flexibility of TGE has allowed this technique to be widely adopted in early phase discovery. There are two main bottlenecks in protein production that need to be addressed to make TGE profitable: increasing protein production yield and improving protein recovery yield.

To achieve high transient protein yields, TGE is dependent on an efficient transfection method. In this application note we review a protein production system, named FectoCHO® Expression System from Polyplus-transfection® for optimal protein production in several commercially available suspension CHO cell lines.

To recover the target protein, we chose flowthrough (FT) purification technologies, as they improve the throughput by reducing time and facilitating the operation. With MabXpure™, a novel truly single-use chromatography media, we increased recovery yield by combining size exclusion chromatography (SEC) and anion exchange (AEX).